Differing caspase-1 activation states in monocyte versus macrophage models of IL-1beta processing and release

Journal of Leukocyte Biology
J Michelle Kahlenberg, George R Dubyak

Abstract

The release of IL-1beta as an active, mature cytokine requires proteolytic processing by caspase-1, which is recruited to signaling complexes that facilitate its autocatalytic proteolysis and activation. Caspase-1 processing has been characterized in human monocyte and murine macrophage model systems, and comparative analyses indicate significant mechanistic differences in caspase-1 activation by these cell types. In this study, we used an in vitro processing assay to compare caspase-1 activation in THP-1 human monocytes vs. Bac1.2F5 murine macrophages. These in vitro caspase-1 and IL-1beta processing reactions indicated a higher rate of constitutive caspase-1 activation in lysates from THP-1 vs. Bac1 cells. Transfer of small amounts of THP-1 lysate to Bac1 lysate rapidly increased in vitro procaspase-1 and proIL-1beta processing in the latter preparation. The transferable activation factor(s) was heat-labile, > or =10 kDa, and unaffected by immunodepletion of procaspase-1 from the THP-1 lysate. This transactivating effect of THP-1 lysate on processing in Bac1 lysates could be mimicked by addition of purified recombinant human caspase-1. The constitutive caspase-1 and IL-1beta processing reactions in THP-1 lysates were insensit...Continue Reading

References

May 31, 1996·The Journal of Biological Chemistry·T T YaminD K Miller
Nov 22, 1996·The Journal of Biological Chemistry·D G PerregauxC A Gabel
Aug 26, 1998·Current Biology : CB·M ThomeJ Tschopp
Dec 9, 1998·The American Journal of Physiology·D G Perregaux, C A Gabel
Sep 14, 1999·British Journal of Pharmacology·C B GrahamesP P Humphrey
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·I WalevS Bhakdi
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D G PerregauxC A Gabel
Jun 8, 2001·The Journal of Biological Chemistry·J L PoyetE S Alnemri
Dec 1, 2001·Immunity·A MacKenzieA Surprenant
Apr 23, 2002·The Journal of Biological Chemistry·Srinivasa M SrinivasulaEmad S Alnemri
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarah J RoweMoira K B Whyte
Dec 3, 2002·Biochemical and Biophysical Research Communications·Nam Jin YooSug Hyung Lee
Mar 28, 2003·American Journal of Physiology. Cell Physiology·Lalitha GudipatyGeorge R Dubyak
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ronald SluyterJames S Wiley
Apr 13, 2004·American Journal of Physiology. Cell Physiology·J Michelle Kahlenberg, George R Dubyak

❮ Previous
Next ❯

Citations

Jul 1, 2010·Journal of Clinical Immunology·Chengcheng Jin, Richard A Flavell
Aug 27, 2010·Plant Molecular Biology·Maurice BoschVernonica E Franklin-Tong
Jul 17, 2009·The Journal of Biological Chemistry·Andrea BabelovaLiliana Schaefer
Sep 21, 2011·The Journal of Biological Chemistry·Maninjay K AtianandJonathan A Harton
Jul 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Dennis C KoSamuel I Miller
Mar 8, 2017·Journal of Immunological Methods·Martha O'BrienDan Lazar
Apr 8, 2017·Molecular and Cellular Biochemistry·Anastasia FamiltsevaSuresh C Tyagi
Sep 6, 2013·Journal of Lipid Research·Laurent L'hommeSylvie Legrand-Poels
Jan 6, 2009·Immunological Reviews·Luigi FranchiGabriel Nuñez
Jul 19, 2012·Biochemistry. Biokhimii︠a︡·Y Sh Schwartz, A V Svistelnik
Jun 12, 2012·Critical Reviews in Biochemistry and Molecular Biology·Jennifer K Dowling, Luke A J O'Neill
Aug 15, 2014·British Journal of Pharmacology·S M KrishnanG R Drummond
Sep 22, 2019·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Takeshi IyonagaHiroyuki Tsutsui
Nov 3, 2016·American Journal of Physiology. Endocrinology and Metabolism·Nicolas J PillonAmira Klip
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mikhail A GavrilinMark D Wewers
Dec 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicolas MolnarfiDanielle Burger
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·J Michelle KahlenbergGeorge R Dubyak
Dec 26, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·J Michelle KahlenbergMariana J Kaplan
Mar 4, 2015·Glia·Christiane MenzfeldUwe-Karsten Hanisch
Mar 23, 2013·Protein & Cell·Shuzhen Chen, Bing Sun
Aug 5, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Clara Westwell-RoperC Bruce Verchere
Jun 13, 2019·International Journal of Hypertension·Daniela Maria TanaseMariana Floria

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.