Difficulties in producing antibodies to purified Propionibacterium acnes exocellular enzymes

The British Journal of Dermatology
E InghamW J Cunliffe


Attempts were made to produce antisera to purified preparations of Propionibacterium acnes lipase, hyaluronate lyase and acid phosphatase in rabbits. Antiserum to lipase (neutralizing titre I:32) was produced using conventional methods. Lipase (30 micrograms) in Freund's complete adjuvant (FCA) was injected into multiple sites thrice at weekly intervals. Antibody levels were boosted by i.v. injections of 30 micrograms in saline at 2-weekly intervals for 2 months. Such regimes failed to raise antibodies to hyaluronate lyase and acid phosphatase. In order to produce antiserum to hyaluronate lyase, rabbit mononuclear cells were incubated with 45 micrograms of hyaluronate lyase in vitro (I h at 37 degrees C) prior to injection subcutaneously above the rabbit ankle joint. Simultaneous injections of hyaluronate lyase in FCA (45 micrograms) were administered into the footpad and thigh. Three treatments at weekly intervals produced an antiserum with a neutralizing titre of I:256. A similar regime failed to raise antibodies to acid phosphatase. Antibodies to P. acnes lipase and hyaluronate lyase also neutralize the enzymes produced by P. granulosum. The neutralization curves suggest that the enzymes produced by both species are related ...Continue Reading


Jul 1, 1978·The British Journal of Dermatology·G GowlandW J Cunliffe
Mar 1, 1977·Infection and Immunity·G WolbergJ K Whisnant
Mar 1, 1974·The Journal of Investigative Dermatology·A M Kligman
Aug 1, 1974·Journal of Medical Microbiology·R R Marples, K J McGinley
Oct 1, 1981·The Journal of Applied Bacteriology·K T HollandW J Cunliffe
Jun 1, 1981·Journal of General Microbiology·E InghamW J Cunliffe


Apr 1, 1986·Applied Biochemistry and Biotechnology·R J LinhardtC L Cooney
Jan 1, 1985·The International Journal of Biochemistry·R J LinhardtD W Persinger

Related Concepts

Percutaneous Coronary Intervention for ST Elevation Myocardial Infarction-Unstable-Over 12 Hours From Symptom Onset
Acid Phosphatase
Antibodies, Bacterial
Neutralization Tests
Propionibacterium acnes

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.


Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.