Diffuse and persistent blood-spinal cord barrier disruption after contusive spinal cord injury rapidly recovers following intravenous infusion of bone marrow mesenchymal stem cells

Experimental Neurology
Takashi MatsushitaJeffery D Kocsis

Abstract

Intravenous infusion of mesenchymal stem cells (MSCs) has been shown to reduce the severity of experimental spinal cord injury (SCI), but mechanisms are not fully understood. One important consequence of SCI is damage to the microvasculature and disruption of the blood spinal cord barrier (BSCB). In the present study we induced a contusive SCI at T9 in the rat and studied the effects of intravenous MSC infusion on BSCB permeability, microvascular architecture and locomotor recovery over a 10week period. Intravenously delivered MSCs could not be identified in the spinal cord, but distributed primarily to the lungs where they survived for a couple of days. Spatial and temporal changes in BSCB integrity were assessed by intravenous infusions of Evans blue (EvB) with in vivo and ex vivo optical imaging and spectrophotometric quantitation of EvB leakage into the parenchyma. SCI resulted in prolonged BSCB leakage that was most severe at the impact site but disseminated extensively rostral and caudal to the lesion over 6weeks. Contused spinal cords also showed an increase in vessel size, reduced vessel number, dissociation of pericytes from microvessels and decreases in von Willebrand factor (vWF) and endothelial barrier antigen (EBA)...Continue Reading

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Citations

Feb 13, 2016·Frontiers in Cellular Neuroscience·Andrea TrostHerbert A Reitsamer
Jun 3, 2017·The Journal of Spinal Cord Medicine·Laureen D HachemMichael G Fehlings
Jan 24, 2018·Journal of Neurotrauma·Jean-Claude StamegnaFrançois S Roman
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May 10, 2018·Frontiers in Neuroscience·Nan-Fu ChenWu-Fu Chen
May 19, 2020·Expert Opinion on Biological Therapy·Simonetta PapaFilippo Rossi
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Feb 10, 2021·Cell Transplantation·Liyi HuangQuan Wei
Nov 19, 2020·Stem Cells International·Liansheng GaoGao Chen
Dec 10, 2020·Journal of Neurotrauma·Lin-Yu JinHai-Ying Liu
Jan 6, 2021·Cytokine & Growth Factor Reviews·Inês BarrosCatarina Oliveira Miranda
May 18, 2021·Frontiers in Physiology·Chun YaoBin Yu

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