Diffuse colonies of human skin fibroblasts in relation to cellular senescence and proliferation

Aging
Vadim ZorinAndreyan N Osipov

Abstract

Development of personalized skin treatment in medicine and skin care may benefit from simple and accurate evaluation of the fraction of senescent skin fibroblasts that lost their proliferative capacity. We examined whether enriched analysis of colonies formed by primary human skin fibroblasts, a simple and widely available cellular assay, could reveal correlations with the fraction of senescent cells in heterogenic cell population. We measured fractions of senescence associated β-galactosidase (SA-βgal) positive cells in either mass cultures or colonies of various morphological types (dense, mixed and diffuse) formed by skin fibroblasts from 10 human donors. Although the donors were chosen to be within the same age group (33-54 years), the colony forming efficiency of their fibroblasts (ECO-f) and the percentage of dense, mixed and diffuse colonies varied greatly among the donors. We showed, for the first time, that the SA-βgal positive fraction was the largest in diffuse colonies, confirming that they originated from cells with the least proliferative capacity. The percentage of diffuse colonies was also found to correlate with the SA-βgal positive cells in mass culture. Using Ki67 as a cell proliferation marker, we further de...Continue Reading

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Citations

Jan 11, 2019·Nanomaterials·Sara Paola Hernández MartínezCristina Rodríguez Padilla
Apr 5, 2018·Stem Cell Research & Therapy·Chunli WangShuyan Chen
Jan 24, 2019·Marine Drugs·Yu Jae HyunJin Won Hyun
Oct 9, 2020·Oxidative Medicine and Cellular Longevity·Izabela Sadowska-Bartosz, Grzegorz Bartosz
Dec 19, 2020·Journal of Neuromuscular Diseases·Sergey N BardakovArtur A Isaev

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Methods Mentioned

BETA
biopsy
biopsies

Software Mentioned

Statistica

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