Diffuse pharmacophoric domains of vasoactive intestinal peptide (VIP) and further insights into the interaction of VIP with the N-terminal ectodomain of human VPAC1 receptor by photoaffinity labeling with [Bpa6]-VIP

The Journal of Biological Chemistry
Yossan-Var TanMarc Laburthe

Abstract

The widespread 28-amino acid neuropeptide vasoactive intestinal peptide (VIP) exerts its many biological effects through interaction with serpentine class II G protein-coupled receptors named VPAC receptors. We previously provided evidence for a physical contact between the side chain at position 22 of VIP and the N-terminal ectodomain of the hVPAC1 receptor (Tan, Y. V., Couvineau, A., Van Rampelbergh, J., and Laburthe, M. (2003) J. Biol. Chem. 278, 36531-36536). We explored here the contact site between hVPAC1 receptor and the side chain at position 6 of VIP by photoaffinity labeling. The photoreactive para-benzoyl-l-Phe (Bpa) was substituted for Phe(6) in VIP resulting in [Bpa(6)]-VIP, which was shown to be a hVPAC1 receptor agonist in Chinese hamster ovary cells stably expressing the recombinant receptor. After obtaining the covalent (125)I-[Bpa(6)-VIP].hVPAC1 receptor complex, it was sequentially cleaved by cyanogen bromide, peptide N-glycosidase F, endopeptidase Glu-C, and trypsin, and the cleavage products were analyzed by electrophoresis. The data demonstrated that (125)I-[Bpa(6)-VIP] were covalently attached to the short 104-108 fragment within the N-terminal ectodomain of the receptor. The data were confirmed by creati...Continue Reading

References

Apr 29, 1994·Biochemical and Biophysical Research Communications·A CouvineauM Laburthe
Oct 12, 2000·Biochemical and Biophysical Research Communications·P NicoleM Laburthe
Jan 2, 2001·The Journal of Biological Chemistry·L LinsM Laburthe
Feb 28, 2002·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M DelgadoJ Leceta

❮ Previous
Next ❯

Citations

Jul 2, 2008·Journal of Molecular Neuroscience : MN·Alain CouvineauMarc Laburthe
Jul 19, 2013·Nature·Fai Yiu SiuRaymond C Stevens
Feb 21, 2007·Biochemistry. Biokhimii︠a︡·E L Vodovozova
Sep 29, 2011·British Journal of Pharmacology·Alain Couvineau, Marc Laburthe
Dec 17, 2016·Chemical Reviews·György DormánGlenn D Prestwich
Feb 1, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Emilie CeraudoAlain Couvineau
Nov 10, 2006·The Journal of Biological Chemistry·Hidefumi ShinoharaYoshikatsu Matsubayashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.