Diffuse retro-reflective imaging for improved video tracking of mosquitoes at human baited bednets.

Royal Society Open Science
Vitaly VoloshinDavid P Towers

Abstract

Robust imaging techniques for tracking insects have been essential tools in numerous laboratory and field studies on pests, beneficial insects and model systems. Recent innovations in optical imaging systems and associated signal processing have enabled detailed characterization of nocturnal mosquito behaviour around bednets and improvements in bednet design, a global essential for protecting populations against malaria. Nonetheless, there remain challenges around ease of use for large-scale in situ recordings and extracting data reliably in the critical areas of the bednet where the optical signal is attenuated. Here, we introduce a retro-reflective screen at the back of the measurement volume, which can simultaneously provide diffuse illumination, and remove optical alignment issues while requiring only one-sided access to the measurement space. The illumination becomes significantly more uniform, although noise removal algorithms are needed to reduce the effects of shot noise, particularly across low-intensity bednet regions. By systematically introducing mosquitoes in front of and behind the bednet in laboratory experiments, we are able to demonstrate robust tracking in these challenging areas. Overall, the retro-reflective...Continue Reading

References

Jun 21, 2003·Optics Letters·Catherine E TowersJulian D C Jones
Jul 16, 2010·Journal of the Royal Society, Interface·Andrew D StrawMichael H Dickinson
Feb 12, 2011·PLoS Medicine·Pedro L AlonsoMarcel Tanner
Jan 19, 2012·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Sachit ButailDerek A Paley
May 26, 2012·Journal of the Royal Society, Interface·Sachit ButailDerek A Paley
Jun 28, 2013·Journal of Medical Entomology·Sachit ButailDerek A Paley
Apr 15, 2016·Journal of the Royal Society, Interface·N C Angarita-JaimesD P Towers
Mar 4, 2018·Parasites & Vectors·Jeroen Spitzen, Willem Takken
Apr 23, 2019·Data in Brief·Sergio BeniniPierangelo Migliorati
Dec 4, 2019·Nature Microbiology·Gregory P D MurrayPhilip J McCall

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Methods Mentioned

BETA
imaging techniques

Software Mentioned

OpenCV

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