Diffusion and binding measurements within oral biofilms using fluorescence photobleaching recovery methods

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
J J BirminghamR Treloar

Abstract

Numerous studies have postulated that bacteria which reside in a biofilm differ from planktonic bacteria. These differences are thought to affect biofilm permeability and, indirectly, the susceptibility of biofilm bacteria to antibacterial agents. In this study fluorescence recovery after photobleaching (FRAP) was used to monitor the diffusion and binding characteristics of a set of size fractionated fluorescein isothiocyanate (FTTC)-conjugated dextrans over small areas (ca. 10 micron) in bacterial biofilms. From these measurements it was straightforward to calculate apparent diffusion rates. Initial studies on the concentration dependence of dextran interaction with planktonic bacteria showed that no irreversible interaction was occurring, however, anomalous faster than free solution diffusion rates were obtained. This phenomenon was modelled using novel analytical and numerical methods which incorporate reversible binding with associated fluorescence changes. Apparent diffusion rates measured in biofilms were highly dependent on biofilm preparation. Sucrose starved biofilms produced an apparent slow-down of two- to fivefold depending on dextran molecular mass and location within the biofilm, indicating that diffusion within t...Continue Reading

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Citations

Dec 29, 2004·Biofouling·Lucie MarcotteMichel Lafleur
Jan 27, 2009·Applied and Environmental Microbiology·Shoji TakenakaPhilip S Stewart
Mar 7, 2003·Applied and Environmental Microbiology·Thomas ThurnheerBernhard Guggenheim
Feb 15, 2002·Advances in Dental Research·J D Rudney
May 3, 2018·FEMS Microbiology Letters·Jagadish SankaranThorsten Wohland
Feb 27, 2015·Reports on Progress in Physics·Nicole BillingsKatharina Ribbeck
Nov 16, 2019·NPJ Biofilms and Microbiomes·Jagadish SankaranThorsten Wohland
Nov 8, 2020·NPJ Biofilms and Microbiomes·Jagadish SankaranThorsten Wohland

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