Diffusion Basis Spectrum and Diffusion Tensor Imaging Detect Hippocampal Inflammation and Dendritic Injury in a Virus-Induced Mouse Model of Epilepsy

Frontiers in Neuroscience
Jie ZhanSheng-Kwei Song

Abstract

Hippocampal CA1 inflammation and dendritic loss are common in epilepsy. Quantitative detection of coexisting brain inflammation and injury could be beneficial in monitoring disease progression and assessing therapeutic efficacy. In this work, we used conventional diffusion tensor imaging (DTI, known to detect axonal injury and demyelination) and a novel diffusion basis spectrum imaging (DBSI, known to detect axonal injury, demyelination, and inflammation) to detect hippocampal CA1 lesions resulting from neuronal dendritic injury/loss and concomitant inflammation in Theiler's murine encephalomyelitis virus (TMEV)-induced seizure mice. Following the cross-sectionalex vivodiffusion magnetic resonance imaging measurements, immunohistochemistry was performed to validate DTI and DBSI findings. Both DTI and DBSI detected immunohistochemistry-confirmed dendritic injury in the hippocampal CA1 region. Additionally, DBSI-derived restricted isotropic diffusion tensor fraction correlated with 4',6-diamidine-2'-phenylindole dihydrochloride (DAPI)-positive nucleus counts, and DBSI-derived fiber fraction correlated with dendrite density assessed by microtubule-associated protein 2 staining. DTI-derived fractional anisotropy (FA) correlated wit...Continue Reading

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Citations

Jan 30, 2020·Frontiers in Human Neuroscience·Amjad SamaraSarah A Eisenstein
Jul 23, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Zezhong YeSheng-Kwei Song
Nov 14, 2020·Brain : a Journal of Neurology·Laetitia DegiorgisLaura-Adela Harsan
Feb 28, 2021·Scientific Reports·Zezhong YeJoshua B Rubin

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Software Mentioned

SNAP
MetaMorph
ITK
DBSI
Matlab
MathWorks
ImageJ

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