PMID: 7547189Apr 1, 1995Paper

Diffusion coefficients of ATP and creatine phosphate in isolated muscle: pulsed gradient 31P NMR of small biological samples

NMR in Biomedicine
M J HubleyT S Moerland

Abstract

Measurements of the intracellular diffusion coefficients (Di) of ATP and creatine phosphate (PCr) in stable, isolated preparations of skeletal muscle were made by means of pulsed field gradient (PFG) 31P NMR. Experiments used a PFG NMR probe specifically designed for small, superfused biological samples. This provided a magnetic field gradient in the z axis of up to 195 G/cm with minimal eddy currents. DiATP and DiPCr in white (fast, glycolytic) skeletal muscle from goldfish (Carassius auratus) were determined to be 2.48 +/- 0.33 and 3.49 +/- 0.33 x 10(-6) cm2/s, respectively, at 25 degrees C and a diffusion time of approximately 19 ms. For comparison with Di values, diffusion coefficients of ATP and PCr also were measured in solutions of ionic composition similar to that of fish muscle cytosol. The in vitro diffusion coefficients of ATP and PCr were 3.54 +/- 0.11 and 5.28 +/- 0.08 x 10(-6) cm2/s, respectively, at 25 degrees C.

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Citations

Jul 11, 1998·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·T Q DuongJ J Neil
Nov 1, 1995·NMR in Biomedicine·K NicolayR M Dijkhuizen
Feb 22, 2001·Annual Review of Physiology·W R Ellington
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Sep 29, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Bettina ReglinAxel R Pries
Aug 3, 2010·The Journal of Experimental Biology·Gerolf GrosThomas Jue
Dec 24, 2010·The Journal of Experimental Biology·Stephen T KinseyRichard M Dillaman
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Sep 9, 2016·Journal of Neurophysiology·Brendan LujanRobert Renden
Dec 27, 2018·Molecular Biology of the Cell·Sachin V SurveAlexander Sorkin
Mar 22, 2007·Physics in Medicine and Biology·Denis Le Bihan
Apr 9, 1999·NMR in Biomedicine·S T KinseyT S Moerland
Aug 30, 2019·Experimental Physiology·Carly J McCarthyYouko Ikeda
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Mar 14, 2017·Journal of Muscle Research and Cell Motility·C J Barclay
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Nov 13, 2007·Journal of Theoretical Biology·V A SelivanovM Cascante
Oct 4, 2021·Biophysical Journal·Giacomo BartolucciChristoph A Weber

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