Diffusion coefficients of several rhodamine derivatives as determined by pulsed field gradient-nuclear magnetic resonance and fluorescence correlation spectroscopy.

Journal of Fluorescence
P-O GendronKevin J Wilkinson

Abstract

Rhodamine derivatives are popular, photostable fluorophores that are used in a number of fluorescent based techniques, including fluorescence correlation spectroscopy (FCS). Indeed, in FCS, both rhodamine 6G (R6G) and rhodamine 110 (R110) are used as calibration standards to determine the dimensions of the instrument confocal volume. In spite of a requirement for precise values of the diffusion coefficients, literature values are scarce and vary over an order of magnitude. In this paper, the diffusion coefficients of four rhodamine fluorophores (rhodamine 6G (R6G), rhodamine B (RB), rhodamine 123 (R123), rhodamine 110 (R110)) were determined by pulsed field gradient nuclear magnetic resonance (PFG-NMR) spectrometry and then validated by comparison with fluorescence correlation spectroscopy. With the objective of validating the FCS calibration, diffusion coefficients of several dextrans and a polystyrene nanoparticle were also determined and compared with literature values or theoretical values that were based upon the Stoke-Einstein equation. The work presented here lead us to conclude that the diffusion coefficients for R6G and R110 have generally been underestimated in the literature. We propose revised values of 4.4x10(-10) ...Continue Reading

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Citations

Sep 24, 2011·The Journal of Biological Chemistry·Liudmila ErokhovaPeter Pohl
Dec 30, 2010·Antimicrobial Agents and Chemotherapy·Zeshi ZhangKevin J Wilkinson
Jun 7, 2011·Biochemistry. Biokhimii︠a︡·I V PerevoshchikovaY N Antonenko
Feb 18, 2010·International Journal of Molecular Sciences·Daniel GranaderoWajih Al-Soufi
Jul 20, 2012·International Journal of Medical Sciences·Klaus BraunWaldemar Waldeck
Dec 10, 2013·Nature Nanotechnology·Igor I VlasovJörg Wrachtrup
Nov 26, 2013·Chemistry and Physics of Lipids·Iyrri L SalveminiP D J Moens
May 17, 2014·European Biophysics Journal : EBJ·T BernasJ Dobrucki
May 2, 2014·Glycobiology·Masamichi NagaeYoshiki Yamaguchi
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Feb 5, 2015·Biophysical Journal·Xiaolu ZhangJason E DeRouchey
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