Diffusion-in-gel enzyme-linked immunosorbent assay for routine detection of IgG and IgM antibodies to Campylobacter jejuni

The Journal of Infectious Diseases
Ake SvedhemB Kaijser

Abstract

A diagnostic serologic test for antibodies to Campylobacter jejuni is presented. A diffusion-in-gel enzyme-linked immunosorbent assay with a surface antigen pool from two C jejuni strains was used. In testing serum samples for antibodies to C jejuni this system possessed high specificity, reliability, sensitivity, and capacity. It is easy to perform and there is no demand for expensive equipment. IgG and IgM antibodies can be quantitated separately, which allows for discrimination between individuals with acute infections and those with antibodies remaining after recovery. One serum sample is often sufficient to obtain such information. Seven groups of individuals were investigated. Serum samples from a total of 159 patients in three different groups and from 306 healthy individuals in four different groups were tested.

Citations

Jun 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P HerbrinkW C van Dijk
Sep 30, 2000·Clinical and Experimental Immunology·S A CawthrawD G Newell
Apr 10, 1999·Letters in Applied Microbiology·P L Connerton, I F Connerton
Sep 1, 1990·The Journal of Applied Bacteriology·P L Griffiths, R W Park
May 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A WalanR Lock
Dec 1, 1991·Annals of the Rheumatic Diseases·T BremellA Svedhem
Apr 1, 1986·Journal of Clinical Pathology·C S GoodwinB J Marshall
Aug 10, 2010·Journal of Inflammation·Natalia MossbergCharlotta Movitz
Jul 17, 2012·Diagnostic Microbiology and Infectious Disease·Katrin Gaardbo KuhnKaren A Krogfelt
Jan 1, 1989·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·J HöökT U Kosunen
Apr 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·B Kaijser
Jun 30, 2004·Scandinavian Journal of Gastroenterology·H StridE S Björnsson
Apr 18, 2012·Acta Neurologica Scandinavica·N MossbergO Andersen
Apr 1, 1987·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·H Rautelin
Oct 1, 1987·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·H Rautelin, T U Kosunen
Jul 1, 1988·Journal of Clinical Microbiology·S D MillsJ L Penner
Sep 25, 1997·Clinical and Diagnostic Laboratory Immunology·T F IsmailL F Peruski
Oct 1, 1987·Journal of Clinical Microbiology·H I Rautelin, T U Kosunen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

Campylobacteriosis

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.