Diffusion of albumins in rat cortical slices and relevance to volume transmission

Neuroscience
L Tao, C Nicholson

Abstract

The apparent diffusion coefficient, D*, was measured in rat cortical slices and compared to the free diffusion coefficient, D, for three negatively charged proteins, lactalbumin (mol. wt = 14,500), ovalbumin (45,000) and bovine serum albumin (66,000). The temporal evolution of the spatial distribution of albumin molecules labeled with the Texas Red fluorophore was determined using integrative optical imaging at intervals after a brief pressure injection from a micropipette in slices of adult rat cerebral cortex and dilute agarose gel. Diffusion coefficients were obtained by fitting appropriate equations to the data. In slices at 34 degrees C, the values of D* (10(-7) cm2/s, mean +/- S.E.M.) for lactalbumin, ovalbumin and bovine serum albumin were 2.37 +/- 0.10, 1.60 +/- 0.08 and 1.63 +/- 0.07, respectively. In agarose gel, values of D (10(-7) cm2/s) were 11.87 +/- 0.20, 10.02 +/- 0.25 and 8.29 +/- 0.17, respectively. From these data the tortuosity factors, (D/D*)0.5, were calculated, with 2.24 obtained for lactalbumin, 2.50 for ovalbumin and 2.26 for bovine serum albumin. Previous optical measurements using dextrans with mol. wts of 40,000 and 70,000 gave tortuosities of 2.16 and 2.25, but in contrast previous determinations wi...Continue Reading

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