Diffusion of myelin oligodendrocyte glycoprotein in living OLN-93 cells investigated by raster-scanning image correlation spectroscopy (RICS).

Journal of Fluorescence
Ellen GielenMarcel Ameloot

Abstract

Many membrane proteins and lipids are partially confined in substructures ranging from tens of nanometers to micrometers in size. Evidence for heterogeneities in the membrane of oligodendrocytes, i.e. the myelin-producing cells of the central nervous system, is almost exclusively based on detergent methods. However, as application of detergents can alter the membrane phase behaviour, it is important to investigate membrane heterogeneities in living cells. Here, we report on the first investigations of the diffusion behavior of the myelin-specific protein MOG (myelin oligodendrocyte glycoprotein) in OLN-93 as studied by the recently developed RICS (raster-scanning image correlation spectroscopy) technique. We implemented RICS on a standard confocal laser-scanning microscope with one-photon excitation and analog detection. Measurements on FITC-dextran were used to evaluate the performance of the system and the data analysis procedure.

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Apr 14, 2010·International Journal of Molecular Sciences·Radek Machán, Martin Hof
May 4, 2013·Annual Review of Plant Biology·Jan MalinskyWidmar Tanner
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Dec 2, 2014·PloS One·Minghao GuoMartin Gruebele
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Jan 25, 2011·Biomedical Optics Express·Kaveh AzartashEnrico Gratton
Mar 12, 2021·Brain : a Journal of Neurology·Caterina MacriniEdgar Meinl

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