Diffusion of radiotracers in normal and ischemic brain slices

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
C S PatlakG C Newman

Abstract

Diffusion in the extracellular space (ECS) is important in physiologic and pathologic brain processes but remains poorly understood. To learn more about factors influencing tissue diffusion and the role of diffusion in solute-tissue interactions, particularly during cerebral ischemia, we have studied the kinetics of several radiotracers in control and hypoxic 450-microm hippocampal slices and in 1,050-microm thick slices that model the ischemic penumbra. Kinetics were analyzed by nonlinear least squares methods using models that combine extracellular diffusion with tissue compartments in series or in parallel. Studies with 14C-polyethylene glycol confirmed prior measurements of extracellular volume and that ECS shrinks during ischemia. Separating diffusion from transport also revealed large amounts of 45Ca that bind to or enter brain as well as demonstrating a small, irreversibly bound compartment during ischemia. The rapidity of 3H2O entry into cells made it impossible for us to distinguish intracellular from extracellular diffusion. The diffusion-compartment analysis of 3-O-methylglucose data appears to indicate that 5 mmol/L glucose is inadequate to support glycolysis fully in thick slices. Unexpectedly, the diffusion coeffi...Continue Reading

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Citations

Jun 10, 2004·Neurochemistry International·Sabina Hrabetová, Charles Nicholson
Oct 17, 2008·Physiological Reviews·Eva Syková, Charles Nicholson
Jan 23, 2013·Fluids and Barriers of the CNS·Irena LoryanMargareta Hammarlund-Udenaes
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Sep 20, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S Hrabetová, C Nicholson
Jan 25, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sabina HrabetováCharles Nicholson
Mar 29, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·George C NewmanKeith W Jones
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Methods Mentioned

BETA
electrophoresis
nuclear magnetic resonance
fluorescence microscopy
imaging techniques

Software Mentioned

Axum
PC
SPSS

Related Concepts

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Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
S Hrabetová, C Nicholson
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