Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes.

Photosynthesis Research
Sashka Boychova KrumovaHerbert van Amerongen

Abstract

We investigated the effects of digalactosyl-diacylglycerol (DGDG) on the organization and thermal stability of thylakoid membranes, using wild-type Arabidopsis thaliana and the DGDG-deficient mutant, dgd1. Circular-dichroism measurements reveal that DGDG-deficiency hampers the formation of the chirally organized macrodomains containing the main chlorophyll a/b light-harvesting complexes. The mutation also brings about changes in the overall chlorophyll fluorescence lifetimes, measured in whole leaves as well as in isolated thylakoids. As shown by time-resolved measurements, using the lipophylic fluorescence probe Merocyanine 540 (MC540), the altered lipid composition affects the packing of lipids in the thylakoid membranes but, as revealed by flash-induced electrochromic absorbance changes, the membranes retain their ability for energization. Thermal stability measurements revealed more significant differences. The disassembly of the chiral macrodomains around 55°C, the thermal destabilization of photosystem I complex at 61°C as detected by green gel electrophoresis, as well as the sharp drop in the overall chlorophyll fluorescence lifetime above 45°C (values for the wild type-WT) occur at 4-7°C lower temperatures in dgd1. Simi...Continue Reading

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Citations

Mar 27, 2012·Photosynthesis Research·Ziru LiThomas D Sharkey
Aug 24, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Sin Man Lam, Guanghou Shui
Sep 9, 2015·Biochimie·Krzysztof BaczynskiMarta Pasenkiewicz-Gierula
Dec 18, 2013·Biochimica Et Biophysica Acta·Győző Garab
May 31, 2014·Journal of Photochemistry and Photobiology. B, Biology·Sreedhar NellaepalliLászló Kovács
May 8, 2016·Photosynthesis Research·Václav KarlickýVladimír Špunda
Sep 8, 2018·Plant & Cell Physiology·Kristen Van GelderTariq A Akhtar
Apr 16, 2019·Molecular Plant-microbe Interactions : MPMI·Zaiyong SiYouguo Li
Apr 17, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bruno DeméJuliette Jouhet
May 30, 2017·Photosynthesis Research·Tomas E van den BergRoberta Croce
Dec 15, 2012·Molecular Biology Reports·Qiming WangLiqun Rao
Sep 5, 2019·International Journal of Molecular Sciences·Qi GuoBronwyn J Barkla
Jan 5, 2019·Journal of Bioenergetics and Biomembranes·Nia PetrovaSashka Krumova
Jul 14, 2020·Frontiers in Plant Science·Hamed Sattari VayghanPaolo Longoni
Oct 13, 2018·Biophysical Reports·Xin ShengZhenfeng Liu
Aug 24, 2019·Progress in Lipid Research·Yasuhiro Higashi, Kazuki Saito

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Methods Mentioned

BETA
circular-dichroism

Software Mentioned

Gel
pro analyser
SPCImage

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