Digested Early Preterm Human Milk Suppresses Tumor Necrosis Factor-induced Inflammation and Cytotoxicity in Intestinal Epithelial Cells

Journal of Pediatric Gastroenterology and Nutrition
Yimin ChenGiamila Fantuzzi

Abstract

The aim of this study was to determine the effect of digested whole human milk (HM; first sample available after birth from mothers of premature infants) on inflammation, oxidative stress, and cytotoxicity in Caco-2 human intestinal epithelial cells stimulated with lipopolysaccharides or tumor necrosis factor (TNF) to mimic the potential in vivo insults facing the premature infant's gastrointestinal tract. Fully differentiated Caco-2 cells were exposed to digested HM (n = 10; samples from 10 different individuals) before stimulation with lipopolysaccharides, TNF, or no stimulation overnight. Inflammation was determined by production of interleukin-8, oxidative stress by levels of F2-isoprostane, and cytotoxicity by released lactate dehydrogenase. HM significantly suppressed interleukin-8 production and cytotoxicity in TNF-stimulated cells, while also suppressing cell death under baseline conditions. Individual HM samples differed widely in their ability to modulate cellular responses. Results from this study provide evidence that digested HM can reduce both an exaggerated inflammatory response and intestinal damage that contribute to the pathogenesis of necrotizing enterocolitis.

References

Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E CarioD K Podolsky
Mar 17, 2000·Free Radical Biology & Medicine·L J Roberts, J D Morrow
Mar 30, 2002·The Journal of Biological Chemistry·Maria T AbreuMoshe Arditi
Jan 9, 2003·Archives of Pediatrics & Adolescent Medicine·Lydia FurmanMaureen Hack
Jul 2, 2004·American Journal of Physiology. Cell Physiology·Ryoko MinekawaYuji Murata
Apr 20, 2007·Journal of Perinatology : Official Journal of the California Perinatal Association·P M SiskT M O'Shea
Apr 24, 2007·Free Radical Biology & Medicine·James K FrielVaibhav P Shirwadkar
Aug 22, 2008·Journal of Perinatology : Official Journal of the California Perinatal Association·J Meinzen-DerrE F Donovan
Apr 14, 2010·Journal of Pediatric Gastroenterology and Nutrition·Li YaoWilliam L Diehl-Jones
Apr 22, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jacqueline Van De WalleYves-Jacques Schneider
Nov 15, 2011·Journal of Pediatric Surgery·Cumhur AydemirUgur Dilmen
Dec 15, 2011·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Pamela BachourZiad Abdel-Razzak
Nov 28, 2012·Pediatric Clinics of North America·Olivia Ballard, Ardythe L Morrow
Feb 15, 2014·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Shehzad HudaArun Pramanik
Apr 16, 2014·Pediatric Research·Akhil MaheshwariUNKNOWN Eunice Kennedy Shriver National Institute of Child Health and Human Development Neonatal Research Network
May 21, 2014·Journal of Pediatric Gastroenterology and Nutrition·Alexander H PennGeert W Schmid-Schönbein
Jun 27, 2015·Archives of Gynecology and Obstetrics·Marina DimitrakiGeorgios Galazios
Nov 5, 2015·Advances in Neonatal Care : Official Journal of the National Association of Neonatal Nurses·Riikka IkonenMarja Kaunonen
Aug 19, 2016·The American Journal of Clinical Nutrition·Francesca BraviMonica Ferraroni
Nov 12, 2016·Seminars in Perinatology·Krishnan MohanKumarAkhil Maheshwari
Dec 18, 2016·Seminars in Perinatology·Josef Neu, Mohan Pammi

❮ Previous
Next ❯

Citations

Sep 22, 2018·Clinical Oral Investigations·Layla PanahipourReinhard Gruber

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

JPEN. Journal of Parenteral and Enteral Nutrition
Sascha Meyer, Ludwig Gortner
Acta Paediatrica Academiae Scientiarum Hungaricae
J DénesA Mohácsi
The Journal of Pediatrics
A MIZRAHIW A SILVERMAN
© 2022 Meta ULC. All rights reserved