Abstract
Rapeseed proteins are described to be poorly digestible in chickens. To further identify some molecular locks that may limit their use in poultry nutrition, we conducted a proteomic study on the various chicken digestive contents and proposed an integrative view of the proteins recruited in the crop, proventriculus/gizzard, duodenum, jejunum, and ileum for digestion of rapeseed by-products. Twenty-seven distinct rapeseed proteins were identified in the hydrosoluble fraction of the feed prior ingestion. The number of rapeseed proteins identified in digestive contents decreases throughout the digestion process while some are progressively solubilized in the most distal digestive segment, likely due to a combined effect of pH and activity of specific hydrolytic enzymes. Fifteen chicken proteins were identified in the hydrosoluble proventriculus/gizzard content, including chymotrypsin-like elastase and pepsin. Interestingly, on the 69 distinct proteins identified in duodenum, only 9 were proteolytic enzymes, whereas the others were associated with homeostasis, and carbohydrate, lipid, vitamin and hormone metabolisms. In contrast, chicken proteins identified in jejunal and ileal contents were mostly proteases and peptidases. The pre...Continue Reading
References
Sep 1, 1978·The Journal of Nutrition·D SklanS Hurwitz
Apr 1, 1991·Poultry Science·K Boa-AmponsemP B Siegel
Feb 1, 1986·Poultry Science·D P Rodeheaver, R D Wyatt
Jun 1, 1972·Gut·D J ShearmanD B McClelland
Oct 17, 1983·European Journal of Biochemistry·M Baudys, V Kostka
Sep 1, 1982·Journal of the Science of Food and Agriculture·E J ButlerG R Fenwick
Apr 15, 1995·The Biochemical Journal·K WangL Hood
Nov 5, 1999·The Journal of Biological Chemistry·J Perez-Vilar, R L Hill
Jan 31, 2003·British Poultry Science·B SvihusF Sundby
Apr 1, 1965·Archives of Biochemistry and Biophysics·C A RYAN
Jun 17, 2005·British Poultry Science·K H HuangW L Bryden
Dec 29, 2005·The Journal of Biological Chemistry·Mari KonoRichard L Proia
Jul 16, 2008·Journal of Agricultural and Food Chemistry·Sophie Réhault-GodbertYves Nys
Aug 30, 2008·Poultry Science·J M González-AlvaradoG G Mateos
Nov 29, 2008·Poultry Science·S A AdedokunT J Applegate
Jan 27, 2011·The Journal of Biological Chemistry·Hiroyasu SatoMakoto Murakami
May 15, 2012·Poultry Science·E J KimC M Parsons
Sep 20, 2012·Poultry Science·F Khajali, B A Slominski
Dec 28, 2012·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Paweł BrzękEnrique Caviedes-Vidal
Oct 7, 2014·Journal of Proteomics·Pauline MarieJoël Gautron
Oct 13, 2014·Poultry Science·S N QaisraniW H Hendriks
Jan 13, 2015·Food Chemistry·T J PuumalainenK Vaali
Aug 27, 2016·Journal of Animal Physiology and Animal Nutrition·M M KasprzakJ Wiseman
Nov 12, 2016·Microbial Pathogenesis·Emmanouil Angelakis
Mar 25, 2017·Poultry Science·E RecoulesS Réhault-Godbert