Digestion dynamics in broilers fed rapeseed meal

Scientific Reports
E RecoulesS Réhault-Godbert

Abstract

Rapeseed proteins are described to be poorly digestible in chickens. To further identify some molecular locks that may limit their use in poultry nutrition, we conducted a proteomic study on the various chicken digestive contents and proposed an integrative view of the proteins recruited in the crop, proventriculus/gizzard, duodenum, jejunum, and ileum for digestion of rapeseed by-products. Twenty-seven distinct rapeseed proteins were identified in the hydrosoluble fraction of the feed prior ingestion. The number of rapeseed proteins identified in digestive contents decreases throughout the digestion process while some are progressively solubilized in the most distal digestive segment, likely due to a combined effect of pH and activity of specific hydrolytic enzymes. Fifteen chicken proteins were identified in the hydrosoluble proventriculus/gizzard content, including chymotrypsin-like elastase and pepsin. Interestingly, on the 69 distinct proteins identified in duodenum, only 9 were proteolytic enzymes, whereas the others were associated with homeostasis, and carbohydrate, lipid, vitamin and hormone metabolisms. In contrast, chicken proteins identified in jejunal and ileal contents were mostly proteases and peptidases. The pre...Continue Reading

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Mar 25, 2017·Poultry Science·E RecoulesS Réhault-Godbert

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Datasets Mentioned

BETA
AAK07609.1

Methods Mentioned

BETA
Assay
zymography
acetylation

Software Mentioned

Scaffold
Chordata
Proteome
Proteome Discoverer
Scaffold Q +
MASCOT
R
RStudio
Chromeleon

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