Digestion of chromatin with deoxyribonuclease II

Molecular Biology Reports
M Yaneva, G Dessev

Abstract

The action of DNAse II on DNA in chromatin was studied. The formation of acid-soluble products followed a two-phase kinetic curve. At the end of the first more rapid phase about 25% of DNA was degraded. Early in the degradation process DNA was converted into double stranded fragments, whose sizes were multiples of about 180 base pairs. As the degradation proceeded these fragments were reduced in size. After denaturation DNA from digested chromatin was resolved into discrete single stranded fractions, exact multiples of a ten-nucleotide length, forming a pattern very similar to that observed with DNAase I.chkova LV

References

Feb 1, 1977·Analytical Biochemistry·M YanevaG Dessev
Jul 15, 1976·European Journal of Biochemistry·M Yaneva, G Dessev
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D K OosterhofR L Rill
May 1, 1973·Analytical Biochemistry·G N Dessev, K Grancharov
Sep 20, 1974·Nature·M Noll
Jun 15, 1974·European Journal of Biochemistry·R E StreeckH G Zachau
Oct 27, 1972·Biochimica Et Biophysica Acta·R J Billing, J Bonner
Jun 5, 1973·Journal of Molecular Biology·G N Dessev, K Grancharov
Jan 27, 1971·Nature: New Biology·R J Clark, G Felsenfeld
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·A E Mirsky
Nov 1, 1974·Nucleic Acids Research·M Noll

Citations

Mar 1, 1977·Molecular Biology Reports·M Yaneva, G Dessev
Jul 1, 1984·Molecular Biology Reports·C IovchevaG Dessev
Mar 12, 2004·Annual Review of Physical Chemistry·G Boato, G G Volpi

Related Concepts

Chromatin
Alkaline DNase
DNA, Neoplasm
SDS-PAGE
Micrococcal Nuclease
Neoplasms, Experimental
Oligodeoxyribonucleotides

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