Digestive duet: midgut digestive proteinases of Manduca sexta ingesting Nicotiana attenuata with manipulated trypsin proteinase inhibitor expression.

PloS One
Jorge A ZavalaIan T Baldwin

Abstract

The defensive effect of endogenous trypsin proteinase inhibitors (NaTPIs) on the herbivore Manduca sexta was demonstrated by genetically altering NaTPI production in M. sexta's host plant, Nicotiana attenuata. To understand how this defense works, we studied the effects of NaTPI on M. sexta gut proteinase activity levels in different larval instars of caterpillars feeding freely on untransformed and transformed plants. Second and third instars larvae that fed on NaTPI-producing (WT) genotypes were lighter and had less gut proteinase activity compared to those that fed on genotypes with either little or no NaTPI activity. Unexpectedly, NaTPI activity in vitro assays not only inhibited the trypsin sensitive fraction of gut proteinase activity but also halved the NaTPI-insensitive fraction in third-instar larvae. Unable to degrade NaTPI, larvae apparently lacked the means to adapt to NaTPI in their diet. However, caterpillars recovered at least part of their gut proteinase activity when they were transferred from NaTPI-producing host plants to NaTPI-free host plants. In addition extracts of basal leaves inhibited more gut proteinase activity than did extracts of middle stem leaves with the same protein content. Although larvae can...Continue Reading

References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R JohnsonC Ryan
Jul 1, 1995·Insect Biochemistry and Molecular Biology·A M PetersonM A Wells
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·M A JongsmaW J Stiekema
May 1, 1994·Insect Biochemistry and Molecular Biology·A M PetersonM A Wells
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·I T Baldwin
Apr 27, 2000·Trends in Ecology & Evolution·M Tatar
May 12, 2001·Molecular Ecology·J Winterer, J Bergelson
Dec 1, 2001·Annual Review of Entomology·Myron P ZaluckiStephen B Malcolm
Dec 1, 2001·Annual Review of Entomology·Caroline S Awmack, Simon R Leather
Apr 6, 2002·Bioorganic & Medicinal Chemistry Letters·Inna PergamentMorris Srebnik
Dec 21, 2002·European Journal of Biochemistry·Mariateresa VolpicellaJules Beekwilder
May 29, 2003·Journal of Insect Physiology·C Bolter, M A. Jongsma
Nov 1, 1961·Archives of Biochemistry and Biophysics·B F ERLANGERW COHEN
Feb 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jorge A ZavalaIan T Baldwin
Feb 26, 2004·Archives of Insect Biochemistry and Physiology·A R LopesW R Terra
Mar 26, 2004·Insect Biochemistry and Molecular Biology·Kristina GrudenMaarten A Jongsma
Mar 15, 2005·Insect Biochemistry and Molecular Biology·Nanasaheb P ChouguleVidya S Gupta
Nov 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alex BayésJosep Vendrell
Nov 11, 2005·The FEBS Journal·John T Christeller
Dec 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hui ChenGregg A Howe
Aug 10, 2006·Cell and Tissue Research·Christian LohrDaniela Hirnet
Oct 31, 2007·Insect Biochemistry and Molecular Biology·Daniela BrioschiMarcio C Silva-Filho

❮ Previous
Next ❯

Citations

Sep 21, 2013·Nature Communications·Ningjia HeZhonghuai Xiang
Dec 19, 2009·Journal of Experimental Botany·Gianni VandenborreEls J M Van Damme
Feb 9, 2012·Journal of Integrative Plant Biology·Tohir Ahmadovich BozorovIan T Baldwin
Nov 16, 2010·Molecular Ecology·Noah K WhitemanNaomi E Pierce
Oct 24, 2014·Annual Review of Entomology·Keyan Zhu-Salzman, Rensen Zeng
Apr 28, 2017·Molecular Ecology Resources·Tobias LortzingAnke Steppuhn
Nov 15, 2020·International Journal of Biological Macromolecules·Hao YanGenhong Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
X-ray

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.