Digital DNA detection based on a compact optofluidic laser with ultra-low sample consumption

Lab on a Chip
Wonsuk LeeDong Ki Yoon

Abstract

DNA lasers self-amplify optical signals from a DNA analyte as well as thermodynamic differences between sequences, allowing quasi-digital DNA detection. However, these systems have drawbacks, such as relatively large sample consumption and complicated dye labelling. Moreover, although the lasing signal can detect the target DNA, it is superimposed on an unintended fluorescence background, which persists for non-target DNA samples as well. From an optical point of view, it is thus not truly digital detection and requires spectral analysis to identify the target. In this work, we propose and demonstrate an optofluidic laser that has a single layer of DNA molecules as the gain material. A target DNA produces intensive laser emission comparable to existing DNA lasers, while any unnecessary fluorescence background is successfully suppressed. As a result, the target DNA can be detected with a single laser pulse, in a truly digital manner. Since the DNA molecules cover only a single layer on the surface of the laser microcavity, the DNA sample consumption is a few orders of magnitude lower than that of existing DNA lasers. Furthermore, the DNA molecules are stained by simply immersing the microcavity in the intercalating dye solution,...Continue Reading

References

Sep 7, 2017·Lab on a Chip·Chaoyang GongXudong Fan
Nov 15, 2018·Micromachines·Zhiqing Feng, Lan Bai
Aug 10, 2017·Optics Express·Wonsuk LeeDong Ki Yoon

Citations

Apr 7, 1988·Nature·R HiguchiH A Erlich
Oct 6, 2000·Physical Review Letters·H J MoonK An
Dec 4, 2002·Journal of Cellular Biochemistry·Huidong ShiTim Hui-Ming Huang
May 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vladimir V Kapitonov, Jerzy Jurka
Aug 9, 2003·Nucleic Acids Research·Véronique Le BerreJean François
Apr 30, 2005·Nature Reviews. Genetics·Robert W Taylor, Doug M Turnbull
Sep 2, 2005·Nature Reviews. Genetics·Keith D Robertson
Nov 23, 2005·The Pharmacogenomics Journal·B S Shastry
Sep 12, 2006·Nature Biotechnology·Roger D CanalesFederico M Goodsaid
Apr 3, 2007·Nature Protocols·Jesse MontgomeryLuming Zhou
Apr 5, 2007·Nature Protocols·Brian M Zeglis, Jacqueline K Barton
Nov 27, 2007·Biosensors & Bioelectronics·Jonathan D SuterXudong Fan
Dec 22, 2007·Chemical Reviews·Audrey SassolasLoïc J Blum
Apr 4, 2008·Nature·Laura J van't Veer, René Bernards
Nov 12, 2007·Optics Express·Scott LaceyXudong Fan
Oct 23, 2009·Nature·Stephen P Jackson, Jiri Bartek
Jun 10, 2010·Nature Reviews. Genetics·R David HawkinsBing Ren
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yuze SunXudong Fan
Sep 29, 2012·Analytical Chemistry·Wonsuk Lee, Xudong Fan
Dec 13, 2012·Chemical Reviews·Hongquan ZhangX Chris Le
Dec 20, 2013·Chemical Society Reviews·Vinalia TjongAshutosh Chilkoti
Feb 1, 2014·Nature Methods·Xudong Fan, Seok-Hyun Yun
Oct 15, 2014·Lab on a Chip·Qiushu ChenXudong Fan
Nov 28, 2014·Sensors·Satish Balasaheb NimseTaisun Kim
Apr 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·David Jablonski, Neil H Shubin
Jun 13, 2015·ACS Applied Materials & Interfaces·Yun Jeong ChaDong Ki Yoon
Nov 20, 2016·Advanced Materials·Yun Jeong Cha, Dong Ki Yoon

Related Concepts

Positron-Emission Tomography
Mass Spectrometry
DNA, A-Form
Analysis
Laser-Evoked Potentials
Pathologic Processes
Non-Target Lesion Identification
Surface
DNA
Detection

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