Digital holographic microscopy long-term and real-time monitoring of cell division and changes under simulated zero gravity

Optics Express
Feng PanWen Xiao

Abstract

The long-term and real-time monitoring the cell division and changes of osteoblasts under simulated zero gravity condition were succeed by combing a digital holographic microscopy (DHM) with a superconducting magnet (SM). The SM could generate different magnetic force fields in a cylindrical cavity, where the gravitational force of biological samples could be canceled at a special gravity position by a high magnetic force. Therefore the specimens were levitated and in a simulated zero gravity environment. The DHM was modified to fit with SM by using single mode optical fibers and a vertically-configured jig designed to hold specimens and integrate optical device in the magnet's bore. The results presented the first-phase images of living cells undergoing dynamic divisions and changes under simulated zero gravity environment for a period of 10 hours. The experiments demonstrated that the SM-compatible DHM setup could provide a highly efficient and versatile method for research on the effects of microgravity on biological samples.

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Citations

Feb 21, 2013·Computational and Mathematical Methods in Medicine·Yunxin WangXinlong Wang
Feb 1, 2015·Journal of Structural Biology·Zahra El-SchichAnette Gjörloff Wingren
Sep 11, 2014·PloS One·Maria Falck MiniotisAnette Gjörloff Wingren

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