Digital holographic microscopy with reduced spatial coherence for three-dimensional particle flow analysis

Applied Optics
Frank DuboisOlivier Monnom

Abstract

We investigate the use of a digital holographic microscope working in partially coherent illumination to study in three dimensions a micrometer-size particle flow. The phenomenon under investigation rapidly varies in such a way that it is necessary to record, for every camera frame, the complete holographic information for further processing. For this purpose, we implement the Fourier-transform method for optical amplitude extraction. The suspension of particles is flowing in a split-flow lateral-transport thin separation cell that is usually used to separate the species by their sizes. Details of the optical implementation are provided. Examples of reconstructed images of different particle sizes are shown, and a particle-velocity measurement technique that is based on the blurred holographic image is exploited.

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Citations

Oct 5, 2010·Micron : the International Research and Review Journal for Microscopy·Yevgeny BeidermanZeev Zalevsky
Jan 6, 2009·Annals of the New York Academy of Sciences·Andreia PedregalJohn Wérenne
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Jun 7, 2011·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Chandra Sekhar SeelamantulaMichael Unser
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Jan 27, 2010·Analytical Chemistry·Claire Ratier, Mauricio Hoyos

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