Digital image analysis and identification of eggs from bovine parasitic nematodes

Journal of Helminthology
C Sommer

Abstract

Computer-assisted microscopy and multivariate statistics were used to establish and evaluate a procedure for identification of bovine strongylid eggs. Ostertagia ostertagi, Cooperia oncophora, Haemonchus placei, Trichostrongylus axei, and Oesophagostomum radiatum eggs were obtained from faeces voided by monospecifically infected calves. Images of single eggs (400 x magnification) were recorded by a CCD camera fitted onto a microscope and digitized on a PC. After separation of eggs from the image background, the pixel (picture element) positions of the egg outline were analysed by algorithms to describe size and shape. A stepwise discriminant analysis was subsequently used to select and rank descriptive features of 4207 eggs according to discriminatory power. Classification criteria were developed by linear discrimination analysis on the basis of selected features, and the criteria evaluated by cross-validation. A maximum average percentage of correct classification of 85.8% resulted when nineteen features were employed in a linear classification criterion. The percentages correct classification for each species were: O. ostertagi 76.3%, C. oncophora 90.8%, O. radiatum 87.8%, H. placei 90.1%, and T. axei 83.8%. Classification ba...Continue Reading

References

Nov 1, 1953·Parasitology·G CUNLIFFE, H D CROFTON
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Jan 1, 1986·IEEE Transactions on Pattern Analysis and Machine Intelligence·S R Dubois, F H Glanz

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Citations

Apr 15, 2000·Computer Methods and Programs in Biomedicine·G TheodoropoulosB Martinez-Gonzales
Apr 6, 1999·Veterinary Parasitology·A DaugschiesW Bollwahn
Sep 25, 1999·Veterinary Parasitology·A PlittA Daugschies
Aug 19, 2014·Computers in Biology and Medicine·Johan Musaeus BruunChristian M O Kapel
May 11, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kathrin A SimNeil B Chilton
Aug 16, 2019·PloS One·Timothy J LyonsJeffrey E Hill
Mar 20, 2020·Parasites & Vectors·Edward King'oriSamer Angelone
May 30, 2021·Veterinary Parasitology·Ramanathan K Kasimanickam, Vanmathy R Kasimanickam
Jul 5, 2021·Veterinary Parasitology·Martin K Nielsen

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