Digital integration of granulocyte aggregation responses. A simple and reproducible method for the quantitation of granulocyte adhesiveness

Inflammation
P R CraddockD E Hammerschmidt

Abstract

The pulmonary leukostasis and lung dysfunction associated with intravascular complement activation results from C5a-mediated granulocyte (GR) aggregation, a phenomenon which can be reproduced in vitro using standard nephelometric techniques. To produce a more subjective measure of the extent and rate of GR aggregation responses we added a digital integrator to the system. The validity of this approach was substantiated by the close correlation between the aggregating and chemotactic activities of C5a and N-formyl-methionine-leucine-phenylalanine. Use of this technique enabled us to define the dose-response relationship of the aggregation produced by the cationophore A23187 and the inhibitory effect of tetracaine on divalent cation-dependent aggregation responses. The aggregation produced by these three stimuli does not result primarily from simple cross-linking of surface changes because, unlike the passive cell-cell association produced by the cation poly-L-lysine, it is not inhibited by anionic poly-L-glutamic acid. The importance of microtubules as regulators of GR adhesiveness was substantiated by the inhibitory effects of colchicine (but not lumicolchicine) on aggregation in this system. These data suggest that this integ...Continue Reading

References

Mar 1, 1978·The Journal of Surgical Research·J R Fletcher, P W Ramwell
Apr 7, 1977·The New England Journal of Medicine·P R CraddockH S Jacob
Jun 20, 1974·The New England Journal of Medicine·P R CraddockH S Jacob
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J M OliverR D Berlin
Apr 18, 1972·Biochimica Et Biophysica Acta·D Papahadjopoulos
Aug 1, 1974·Infection and Immunity·A L SmithD H Smith
Sep 26, 1974·The New England Journal of Medicine·R R MacGregorA L Lentnek

❮ Previous
Next ❯

Citations

Dec 1, 1981·Inflammation·D J SchmelingP K Peterson
Sep 1, 1982·Inflammation·W W BullockW L Gabler
Sep 1, 1983·Respiration Physiology·E K WeirP R Craddock
Jan 1, 1983·Pharmacology & Therapeutics·S Massa, H B Bosmann
Sep 13, 1982·Biochimica Et Biophysica Acta·H B KaplanG Weissmann
Jan 1, 1990·Archives of Oral Biology·S KrauseP Gängler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.