Digital Microarrays: Single-Molecule Readout with Interferometric Detection of Plasmonic Nanorod Labels

ACS Nano
Derin SevenlerM Selim Unlü

Abstract

DNA and protein microarrays are a high-throughput technology that allow the simultaneous quantification of tens of thousands of different biomolecular species. The mediocre sensitivity and limited dynamic range of traditional fluorescence microarrays compared to other detection techniques have been the technology's Achilles' heel and prevented their adoption for many biomedical and clinical diagnostic applications. Previous work to enhance the sensitivity of microarray readout to the single-molecule ("digital") regime have either required signal amplifying chemistry or sacrificed throughput, nixing the platform's primary advantages. Here, we report the development of a digital microarray which extends both the sensitivity and dynamic range of microarrays by about 3 orders of magnitude. This technique uses functionalized gold nanorods as single-molecule labels and an interferometric scanner which can rapidly enumerate individual nanorods by imaging them with a 10× objective lens. This approach does not require any chemical signal enhancement such as silver deposition and scans arrays with a throughput similar to commercial fluorescence scanners. By combining single-nanoparticle enumeration and ensemble measurements of spots when...Continue Reading

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Citations

Jul 18, 2019·Lab on a Chip·Yoshihiro MinagawaHiroyuki Noji
Oct 1, 2019·Advanced Materials·Yanfang WuJohn Justin Gooding
Feb 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Derin SevenlerM Selim Ünlü
Sep 11, 2019·Proceedings of the National Academy of Sciences of the United States of America·Taylor D CanadyBrian T Cunningham
Oct 27, 2020·Advanced Biosystems·Katherine S YangCesar M Castro
Dec 2, 2020·Optics Letters·Celalettin Yurdakul, M Selim Ünlü
Mar 21, 2021·Nature Communications·Nantao LiBrian T Cunningham
Sep 30, 2020·Journal of the American Chemical Society·Dorota GrzelakLuis M Liz-Marzán
Sep 28, 2021·Chemical Reviews·Jiapeng ZhengJianfang Wang
Sep 30, 2021·Chemical Reviews·Lee PriestPhilipp Kukura

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