Digital separation of diaminobenzidine-stained tissues via an automatic color-filtering for immunohistochemical quantification

Biomedical Optics Express
Rong FuJianhua Ma

Abstract

The digital separation of diaminobenzidine (DAB)-stained tissues from hematoxylin background is an important pre-processing step to analyze immunostains. In most stain separation methods, specific color channels (for example: RGB, HSI, CMYK) or color deconvolution matrices are used to obtain different tissue contrasts between DAB- and hematoxylin-stained areas. However, these methods could produce incomplete separation or color changes because the color spectra of stains and co-localized stains overlap in histological images. Therefore, we proposed an automatic color-filtering to separate hematoxylin- and DAB-stained tissues. In implantation, the RGB images of DAB-labeled immunostains are first converted to 8-bit BN images by a mathematical translation to produce the largest contrast between brown DAB-stained tissues and blue hematoxylin-stained tissues. The first valley in the histogram revised by nonuniform quantization is set as the cut-off point to obtain a brown filter. DAB-stained tissues are accurately delineated from the background counterstain, resulting in DAB-only-image and De-DAB-image. Subsequently, a blue filter is designed in the CIE-Lab color space to further delineate the hematoxylin-stained tissues from the De...Continue Reading

References

Apr 22, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Eric M BreyCharles W Patrick
Dec 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Antonio C WolffUNKNOWN College of American Pathologists
Mar 1, 2007·Diagnostic Pathology·Nhu-An PhamDavid W Hedley
Apr 3, 2010·Computer Methods and Programs in Biomedicine·S Di CataldoE Macii
Oct 16, 2010·Journal of Microscopy·P J Tadrous
Jan 20, 2011·IEEE Transactions on Bio-medical Engineering·Elisa FicarraEnrico Macii
Apr 23, 2011·Breast Cancer Research and Treatment·Anja BrügmannMogens Vyberg
Sep 18, 2012·Computers in Biology and Medicine·Santa Di CataldoEnrico Macii
Jul 13, 2014·Methods in Molecular Biology·Clive R Taylor

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Citations

Feb 6, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Abdulwarith O AkinshipoSunday O Akintoye
Feb 16, 2019·Transplantation·Michelle A Wood-TrageserAnthony J Demetris
Nov 22, 2020·Cell Biology and Toxicology·Karthikeyan RajamaniNatarajan Aravindan

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