PMID: 2490762Jan 1, 1989Paper

Digoxin-like immunoreactive substance: monoclonal and polyclonal RIA and FPIA compared

Journal of Pharmaceutical and Biomedical Analysis
P MojaverianG D Chase

Abstract

Digoxin-like immunoreactive substance (DLIS) was measured in 34 samples obtained from subjects not receiving digoxin: 10 uremic, 10 third trimester pregnancy, seven cord blood and seven normal. DLIS concentration was measured by four commercial polyclonal radioimmunoassay (RIA) systems: Clinical Assay (CA), Corning Immophase (CI), Diagnostic Products Double Antibody (DP), Kallestad Quantitope (KK), a monoclonal antibody (MA) assay and a Fluorescence Polarization Immunoassay (FPIA). In general, the cord blood samples were richer in DLIS. Digoxin immunoassays, MA and DP showed minimal interference by DLIS in all samples, whereas FPIA and CA exhibited the maximal cross-reactivity with DLIS. In cord blood samples, mean +/- SD DLIS concentration ranged from 0.41 +/- 0.13 (by CA) to 0.034 +/- 0.02 ng ml-1 as measured by MA assay. In uremics, the mean DLIS concentration was below the detection limit of all RIA assays. The FPIA method showed a higher degree of cross-reactivity to DLIS, especially in the cord and pregnancy samples (0.42 +/- 0.13 and 0.4 +/- 0.14 ng ml-1, respectively). DLIS in uremics was below the FPIA detection limit of 0.2 ng ml-1. Overall, the degree of interference by DLIS in decreasing order was FPIA greater than ...Continue Reading

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Citations

May 29, 2003·Critical Care Medicine·Elmar BerendesMichael Walter

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