Dihydroartemisinin suppresses glycolysis of LNCaP cells by inhibiting PI3K/AKT pathway and downregulating HIF-1α expression.

Life Sciences
Wenhe ZhuHuiyan Wang

Abstract

Dihydroartemisinin (DHA) exhibits potential anticancer activity. However, the biological functions of DHA in prostate cancer remain largely unexplored. In this study, we aim to investigate the anti-proliferative effect and glycolysis regulation of DHA on prostate cancer cell LNCaP. Cell proliferative activity and apoptosis inducing were detected. The gene expression was detected by mRNA microarray and results were analyzed by GO and KEGG pathway database. Expressions of glycolysis key enzymes and PI3K/AKT/HIF-1α were detected by Western blot. Results indicated that DHA could inhibit the LNCaP cell proliferation considerably and induce cell apoptosis. mRNA microarray showed 1293 genes were upregulated and 2322 genes were downregulated. GO and KEGG enrichment analysis suggested that glycolysis pathway was correlated with DHA inhibited the proliferation on the LNCaP cell. Western blot results showed that DHA can decrease GLUT1 and regulatory enzymes of glycolytic pathway expression probably by suppressing the activity of the intracellular Akt/mTOR and HIF-1 α. Experimental validation results indicate that DHA treatment can inhibit the LNCaP cell proliferation and induce apoptosis, which may be related to glycolysis inhibition.

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Citations

Sep 24, 2020·Journal of Biomolecular Structure & Dynamics·Ibrahim MalamiImaobong C Etti
Aug 9, 2020·OncoTargets and Therapy·Wei HanWei Guan
Sep 26, 2020·Cell Death & Disease·Rui LiuYuanyan Liu
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Jun 21, 2021·Medicinal Research Reviews·Yuqing MengJigang Wang
Jul 1, 2021·Drug Design, Development and Therapy·Haoyang HuangXuerong Li
Jul 15, 2021·Acta Biochimica Et Biophysica Sinica·Mingrui JiangDaorong Wang

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