Dihydroartemisinin suppresses the proliferation of Epstein-Barr virus-associated gastric carcinoma cells via downregulation of latent membrane protein 2A

Oncology Letters
Wei GongQiang Liu

Abstract

Treatment of recurrent and metastatic Epstein-Barr virus-associated gastric carcinoma (EBVaGC) remains a challenge, particularly in developing countries, due to lack of efficient screening programs. Latent membrane protein 2A (LMP2A) has been reported to serve an important function in the development of EBVaGC. In previous years dihydroartemisinin (DHA), traditionally used as an anti-malarial agent, has been demonstrated to inhibit tumor growth with low toxicity to normal cells. In the present study, the anti-tumor effect of DHA in EBVaGC was investigated. The MTT assay was used to compare the viability of untreated and DHA-treated EBVaGC GT-38 cells. Flow cytometry was applied to determine the percentage of GT-38 cells at each stage of the cell cycle. Reverse transcription-polymerase chain reaction and western blotting were used to determine the expression of the LMP2A gene. The effect of DHA treatment in vivo was evaluated in nude mice bearing GT-38 tumors. The results of the present study revealed that DHA-treated cells exhibited a time- and dose-dependent inhibition of viability. DHA significantly increased the apoptotic rate of GT-38 cells following treatment with 20 µg/ml DHA for 48 h. DHA-treated GT-38 cells were blocked...Continue Reading

References

Jul 1, 1992·The Journal of Infection·E M RabadãoA A Meliço-Silvestre
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·C L MillerR Longnecker
Feb 9, 2000·Life Sciences·V DhingraM Lakshmi Narasu
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 19, 2005·World Journal of Gastroenterology : WJG·Bing LuoPeng Zhao
Feb 21, 2006·World Journal of Gastroenterology : WJG·Katherine D Crew, Alfred I Neugut
Apr 14, 2007·Mini Reviews in Medicinal Chemistry·Seokjoon Lee
Jun 26, 2007·Acta Pharmacologica Sinica·Yang JiaoSai-jun Fan
Jan 30, 2010·Chemical Society Reviews·Devdutt ChaturvediParuchuri G Rao
Aug 3, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Haijun SunXin Zhang
Jun 11, 2014·World Journal of Gastroenterology : WJG·Tung On YauJun Yu

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.