PMID: 7011812Feb 1, 1981Paper

Dihydrolipoamide dehydrogenase component of the pyruvate dehydrogenase complex from Escherichia coli K12. Comparative characterization of the free and the complex-bound component

European Journal of Biochemistry
E Schmincke-Ott, H Bisswanger

Abstract

The regulation of the biosynthesis of dihydrolipoamide dehydrogenase is dependent on the biosynthesis of the pyruvate dehydrogenase complex. The gene coding for the dihydrolipoamide dehydrogenase appears to be included in the regulation of the pyruvate dehydrogenase operon. Possibly a secondary promoter is inserted. Dihydrolipoamide dehydrogenase was purified in its free form using a dihydrolipoamide-agarose affinity column and avoiding denaturating conditions. The enzyme shows complete cross-reactivity with antibodies against the pyruvate dehydrogenase complex and has a higher specific activity than any preparations described thus far. The transition state activation energy of the catalytic activity is smaller for the complex-bound enzyme than that found for the free enzyme. In its complexed form, the enzyme also proves to be somewhat more stable under alkaline conditions. The reaction catalyzed by the dihydrolipoamide dehydrogenase shows the behaviour of a ping-pong mechanism. The Michaelis constants for the substrates NAD and dihydrolipoamide found with the free enzyme are about four times those observed with the enzyme integrated into the native complex. The catalytic reaction of both forms of the dihydrolipoamide dehydroge...Continue Reading

References

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Citations

May 1, 1993·World Journal of Microbiology & Biotechnology·S P SinghP J Rogers
Jan 1, 1980·Preparative Biochemistry·E Schmincke-Ott, H Bisswanger
Apr 9, 2008·Bioscience, Biotechnology, and Biochemistry·Saikat ChakrabortyKazuo Sakka
Aug 25, 2001·Analytical Biochemistry·G GeigerF Vitzthum
Jan 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·P HeinrichG B Kresze
Sep 29, 2011·Prikladnaia biokhimiia i mikrobiologiia·A Iu SkorokhodovaV G Debabov
Apr 18, 2021·Applied and Environmental Microbiology·W Chris Moxley, Mark A Eiteman

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