PMID: 9540851Apr 16, 1998Paper

Dihydropyridine receptor gene expression in skeletal muscle from mdx and control mice

Biochimica Et Biophysica Acta
Y PéréonPhilip Palade

Abstract

The expression of isoform-specific dihydropyrine receptor-calcium channel (DHPR) alpha 1-subunit genes was investigated in mdx and control mouse diaphragm (DIA) and tibialis anterior (TA). RNase protection assays were carried out with a rat DHPR cDNA probe specific for skeletal muscle and a mouse DHPR cDNA probe specific for cardiac muscle. The level of expression of the gene encoding the cardiac DHPR was very weak in TA muscle from both control and mdx mice. Compared to TA, DIA expressed mRNA for the cardiac isoform at significantly higher levels, but mdx and control mouse DIA levels were similar to one another. In contrast, mRNA expression levels for the DHPR skeletal muscle isoform were lower in control DIA than TA. However, there was a dramatic increase in the expression for the DHPR skeletal muscle isoform in mdx DIA compared with control DIA, reaching the TA expression level, whereas dystrophy did not affect TA expression. [3H]-PN200-110 binding was used to further assess DIA DHPR expression at the protein level. The density of binding sites for the probe was not significantly affected in DIA muscles of mdx vs. control mice, but it was reduced in older mdx and control mice. The increase in DHPR mRNA levels without a conse...Continue Reading

References

Oct 1, 1992·Muscle & Nerve·E E Dupont-Versteegden, R J McCarter
Feb 1, 1989·Muscle & Nerve·W D ReidR L Pardy
May 21, 1996·Biochimica Et Biophysica Acta·M E Kargacin, G J Kargacin

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