Abstract
Dihydrotanshinone (DHT), one of the major ingredients of Salvia miltiorrhiza Bunge (Danshen), displays many bioactivities. However, the activity and underlying mechanism of DHT in anti-inflammation have not yet been elucidated. In this study, we investigated the anti-inflammatory activity and molecular mechanism of action of DHT both in vitro and in vivo. Our data showed that DHT significantly decreased the release of inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, THP-1 cells, and bone marrow-derived macrophages (BMDMs), and altered the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). In addition, flow cytometry results indicated that DHT reduced the calcium influx, and generation of reactive oxygen species (ROS), and nitric oxide (NO) generation in LPS-stimulated RAW264.7 cells. Moreover, DHT suppressed the transcription of nuclear factor-κB (NF-κB), the expressions of NF-κB proteins, and nuclear translocation of NF-κB/p65, thereby suggesting that the NF-κB pathway played a role in the anti-inflammatory action of DHT. In addition, DHT attenuated LPS-challenged activator protein-1 (AP-1) activity, re...Continue Reading
Citations
Mar 28, 2020·Cardiovascular Research·Elena V DolmatovaKathy K Griendling
May 26, 2020·Journal of Experimental & Clinical Cancer Research : CR·Xiaoping WangYong Wang
Aug 6, 2019·Mediators of Inflammation·Yiting ZhangJie Chen
Oct 23, 2019·Scientific Reports·Shan HanShilin Yang
Dec 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jia HeShilin Yang
Sep 11, 2020·Oxidative Medicine and Cellular Longevity·Shasha HeQingquan Liu
Mar 12, 2020·Chinese Medicine·Xin-Xing LiYitao Wang
Jul 7, 2020·Chinese Medicine·Jia HeHongwei Gao
Aug 7, 2020·PloS One·Qin-Qin WangShilin Yang
Dec 19, 2020·Journal of Ethnopharmacology·Zhuangwei ZhangFangmiao Yu
Dec 16, 2020·Antimicrobial Agents and Chemotherapy·Peipei LuoHongkai Bi
Dec 10, 2020·International Journal of Molecular Sciences·Claudia Giuseppina FrestaClaudio Bucolo
Dec 22, 2020·Journal of Immunology Research·Renyikun YuanShilin Yang
Feb 11, 2020·Biochemical and Biophysical Research Communications·Valery M DembitskyVladimir D'yakonov
Mar 9, 2020·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Hongwei GaoShilin Yang
Mar 6, 2021·Drug Design, Development and Therapy·Xinglong LiuZhiqiang Cheng
Oct 1, 2020·International Journal of Molecular Sciences·Seul-Ki MunSung-Tae Yee
Apr 13, 2021·Frontiers in Pharmacology·Yushun CuiShilin Yang
May 1, 2021·Acta Pharmacologica Sinica·Abudumijiti AbuliziBao-Xue Yang
May 13, 2021·Phytotherapy Research : PTR·Xin-Xing LiHongwei Gao
May 21, 2021·The Journal of Organic Chemistry·Maxim R DemidovYuri N Klimochkin
Jun 30, 2021·Chinese Medicine·Youqiong ZhuoHongwei Gao
Jul 3, 2021·Plants·Jing-Hui FengHong-Won Suh
Sep 2, 2021·Inflammation·Jing YueXueshibojie Liu
Aug 31, 2021·World Journal of Gastrointestinal Oncology·Ming-Ming QiWei-Guo Dong
Dec 24, 2021·Frontiers in Pharmacology·Qi- WuYu-Peng Chen