PMID: 32696752Jul 23, 2020Paper

Dihydrotanshinone I (DHTS1) attenuates cuprizone-induced demyelination via regulating microglia polarization

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Fang XuYanhua Li

Abstract

Objective To evaluate whether dihydrotanshinone I (DHTS1) attenuates cuprizone-induced demyelination. Methods DHTS1 was dissolved in 5 g/L sodium carboxymethyl cellulose (CMC-Na). The cuprizone model was induced via feeding with the diet containing 2 g/L cuprizone. We administrated DHTS1 to the cuprizone-exposed mice. The mice were randomly divided into CMC-Na normal group, CMC-Na combined with cuprizone group and DHTS1 combined with cuprizone group. Myelin degeneration was checked by Luxol fast blue (LFB) staining and the immunohistochemical staining of myelin basic protein (MBP) and myelin proteolipid (PLP). Cell apoptosis was measured by TUNEL. Microglia polarization was evaluated by Iba-1, CD86 and CD163 immunohistochemical staining in vivo. The SIM-A9 cells cultured were divided into CMC-Na group, DHTS1 group, CMC-Na combined with LPS group and DHTS1 combined with LPS group. The expression of CD16/32, tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS) was analyzed by flow cytometry in vitro. Results Compared with CMC-Na combined with cuprizone group, DHTS1 treatment significantly attenuated myelin loss and cell apoptosis, reduced the area of Iba-1+ amoebic microglia and the number of CD86+ cells, while...Continue Reading

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