Dihydrotestosterone induces minor transcriptional alterations in genital skin fibroblasts of children with and without androgen insensitivity

Endocrine Journal
Kanako Tanase-NakaoMaki Fukami

Abstract

Endogenous and exogenous androgens induce masculinization of external genitalia through binding to the androgen receptor (AR). The target genes of androgens in external genitalia remain to be determined, although previous studies have shown that the apolipoprotein D gene (APOD) was significantly upregulated by dihydrotestosterone (DHT), the most potent androgen in humans. In the present study, we performed microarray analysis for genital skin fibroblasts obtained from four boys with buried penis (the control individuals) and a patient with partial androgen insensitivity syndrome (PAIS) due to a hypomorphic mutation in AR (the PAIS patient). We identified 24 transcripts that were upregulated or downregulated by DHT in all samples of control individuals and, to a lesser extent, in the sample of the PAIS patient. Differences between DHT-treated and -untreated samples were small; the results of 24 transcripts did not reach statistical significance. The 24 transcripts included CYP1B1, a gene possibly involved in the development of genital tubercle in mice, and APOD, as well as several genes that have been reported as androgen targets in prostate or other tissues. The results of this study indicate that androgen-mediated masculinizat...Continue Reading

References

Jan 1, 1977·British Journal of Plastic Surgery·B S Crawford
Aug 1, 1993·The Biochemical Journal·G JensterA O Brinkmann
Nov 4, 2000·Biochimica Et Biophysica Acta·E RassartR Milne
Jul 11, 2001·Biochemical and Biophysical Research Communications·C S LinT F Lue
Dec 19, 2008·Development·Jing ZhouGuo-Liang Xu
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Mar 31, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Mahesh AppariPaul-Martin Holterhus
Sep 3, 2010·Progress in Molecular Biology and Translational Science·Hiroko Habuchi, Koji Kimata
Oct 12, 2010·PloS One·Ilya KupershmidtMostafa Ronaghi
Jun 28, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Mohanasundari PajaniappanBrenda Lilly
Sep 23, 2011·The Journal of Clinical Endocrinology and Metabolism·A VotteroL Ghizzoni
Feb 16, 2012·Human Mutation·Bruce GottliebMark Trifiro
Oct 18, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rita A Buresh-StiemkePaul C Marker
Nov 16, 2013·Indian Journal of Urology : IJU : Journal of the Urological Society of India·Rajendra B NerliMurigendra B Hiremath
Nov 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Zhengui ZhengMartin J Cohn
Dec 25, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Ivan NalvarteJosé Inzunza
Apr 12, 2016·Molecular and Cellular Endocrinology·David KradolferSusanne E Ulbrich
Nov 5, 2016·The Journal of Clinical Endocrinology and Metabolism·N C HornigP-M Holterhus
Oct 8, 2016·Journal of Ovarian Research·Sylvie JaillardCélia Ravel
Jul 10, 2017·Animal Reproduction Science·Rafik MenadJean-Marie Exbrayat
Dec 22, 2017·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·O V BurmenskayaD Y Trofimov
May 17, 2018·Archives of Endocrinology and Metabolism·Rafael Loch BatistaBerenice Bilharinho de Mendonca
Nov 7, 2018·Anticancer Research·Elisabeth I HeathWael A Sakr

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Citations

Jun 20, 2020·Gene·Eric RassartCatherine Mounier
Oct 26, 2021·Frontiers in Physiology·Diego Sanchez, Maria D Ganfornina

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