DiI staining of fine branches of Bonghan ducts on surface of rat abdominal organs

Journal of Acupuncture and Meridian Studies
B C LeeKwang-Sup Soh

Abstract

Novel thread-like structures and corpuscles, designated Bonghan ducts (BHDs) and corpuscles (BHCs), are known to form a system of networked channels. Here, we tested the effectiveness of a fluorescent carbocyanine dye, DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), in staining BHDs and BHCs. DiI solution was infused into a BHC on the surface of a rat abdominal organ at a steady rate and the resulting labeling of neighboring BHCs connected via BHDs was examined, as identified by the red fluorescence of DiI. BHDs diameters tapered away from BHCs and formed tree-like branches with fine arborizations embedded in the membranous tissues at their terminal parts. In the proximal parts, DiI fluorescence appeared as continuous lines within BHDs, but a large portion of BHDs remained unstained. In the distal parts of BHDs, discontinuous elongated DiI microparticles were identified along the sinuses within BHDs. The results showed that inner spaces within the BHDs allowed DiI to flow and that BHDs have tree-like branches and terminal arborizations. In conclusion, DiI can be used in visualizing BHDs fine structures.

References

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Jun 2, 2004·The Anatomical Record. Part B, the New Anatomist·Byung-Cheon LeeKwang-Sup Soh
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May 18, 2005·The Anatomical Record. Part B, the New Anatomist·Hak-Soo ShinKwang-Sup Soh
Sep 24, 2005·The Anatomical Record. Part B, the New Anatomist·Byung-Cheon LeeKwang-Sup Soh
Oct 5, 2006·Microscopy Research and Technique·Byung-Cheon LeeByung-Soo Chang
Mar 8, 2007·Evidence-based Complementary and Alternative Medicine : ECAM·Hyeon-Min JohngKwang-Sup Soh

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Citations

Aug 13, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Sang Hyun ParkYeon-Hee Ryu
Jun 14, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Hyub HuhSeung Zhoo Yoon
Jul 8, 2015·Stem Cells and Development·Rajani RaiByoung S Kwon
Sep 18, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Chae Jeong LimPan Dong Ryu
Aug 29, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Kwang-Sup SohYeon Hee Ryu
Aug 30, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Chae Jeong LimPan Dong Ryu
Jun 14, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Ding-Jun CaiFan-Rong Liang
Dec 28, 2010·Journal of Acupuncture and Meridian Studies·Tae Hee HanPan Dong Ryu
Jun 28, 2011·Journal of Acupuncture and Meridian Studies·So Rim KimPan Dong Ryu
Aug 10, 2020·Journal of Acupuncture and Meridian Studies·Chae Jeong LimPan Dong Ryu
May 22, 2020·Journal of Acupuncture and Meridian Studies·Linlin Zhang, Sang Wook Oh
Jan 9, 2016·The Journal of the American Osteopathic Association·Bruno ChiklyJörgen Quaghebeur

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