Dilated cardiomyopathy-associated proteins and their presentation in a WWW-accessible two-dimensional gel protein database

Electrophoresis
K P PleissnerE Fleck

Abstract

High resolution two-dimensional electrophoresis (2-DE) and computer-assisted image analysis were used to screen 13 patients suffering from dilated cardiomyopathy (DCM) versus 15 control patients for quantitative and qualitative differences in their myocardial protein expression. Right atrial tissue samples were obtained from end-stage failing explanted hearts and control hearts. Fifty-two spots differed significantly in average intensity between the DCM and the control groups. Myosin light chain 2, ventricular (MLC2) and heat shock protein HSP 27 were identified by protein microsequencing and gel map comparison with other databases. These proteins were found to be characteristic protein markers for DCM in the right atrium. In DCM patients, the spot intensity (protein abundance) of MLC2 is increased to 336% and HSP 27 is decreased to 59%, compared to the control group. The HEART-2DPAGE, a World Wide Web-accessible 2-DE database, was used and extended for the presentation of these disease-associated proteins. Retrievable via Internet we present a list of disease-associated proteins, their altered level of expression in DCM, and their position on a right atrial protein pattern. The accession number to protein sequence databases co...Continue Reading

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Citations

Mar 21, 1998·Electrophoresis·K P PleissnerE Fleck
Sep 26, 2003·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Jun Yoshioka, Richard T Lee
Feb 1, 2000·Drug Discovery Today·M J Dunn
Aug 21, 2003·Human Molecular Genetics·Emma McGregor, Michael J Dunn
May 19, 2009·Journal of Cardiovascular Medicine·Nicola MarzilianoEloisa Arbustini
Nov 30, 2000·Lancet·R E BanksP J Selby
Oct 10, 2007·Journal of Cardiac Failure·Geraldine A FullerPeter J Reiser
Feb 10, 2006·International Journal of Cardiology·Lien LamChristopher Semsarian
Jul 9, 2004·Pathology, Research and Practice·Alicja BukowskaAndreas Goette
Feb 18, 2006·Circulation Research·Emma McGregor, Michael J Dunn

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