Abstract
Hox transcription factors specify body segments along the anteroposterior axis of the embryo. Despite conservation of the homeodomain (HD), different Hox paralogs instruct remarkably different developmental fates. We have unexpectedly found that the Drosophila Sex combs reduced (Scr) protein dimerizes in vivo via the homeodomain, whereas its closest relative, Antennapedia (Antp), does not. Dimerization requires the conserved residue 19 in the ELEKEF motif of the HD and is facilitated by DNA binding. To study Scr dimerization in vivo, we generate a giant transcriptional puff in live salivary gland cells, consisting of a controllable multiple Scr-binding site of the fork head enhancer, and visualize Scr dimer formation upon specific DNA binding. Scr dimerization is required not only for transcriptional activation of the fork head gene but also for Scr homeotic function in the fly (formation of ectopic salivary glands, posterior transformations in the embryo and antenna-to-tarsus transformations). Finally, we attempt to attribute the differential behavior in dimer formation observed between Antp and Scr to diverse amino acid regions between the two proteins that account for dimerization in Scr versus non-dimerization in Antp. By c...Continue Reading
References
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y Q QianK Wüthrich
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M AffolterW J Gehring
Nov 3, 1989·Cell·Y Q QianK Wüthrich
Oct 22, 1982·Science·A C Spradling, G M Rubin
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K Furukubo-TokunagaW J Gehring
Oct 11, 1996·Science·M CallejaG Morata
Jul 10, 1999·Genes & Development·H D Ryoo, R S Mann
Jun 17, 2000·The EMBO Journal·M Berry, W Gehring
Oct 24, 2000·The Journal of Biological Chemistry·H KasaharaS Izumo
Feb 17, 2001·The EMBO Journal·S PlazaW J Gehring
Aug 24, 2001·Developmental Biology·B ZhouS K Beckendorf
May 2, 2002·Molecular Cell·Chang-Deng HuTom K Kerppola
May 3, 2005·Journal of Theoretical Biology·Francisco J P LopesFernando M C Vieira
Oct 26, 2007·Development·Yasushi SakaJames C Smith
Nov 6, 2007·Cell·Rohit JoshiRichard S Mann
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Serge PlazaWalter J Gehring
Feb 10, 2009·Biochemistry·Gabriel BirraneJohn A A Ladias
Aug 28, 2009·Stem Cells·Hailan ZhangMargaret H Baron
Feb 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Vladana VukojevicRudolf Rigler
Feb 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Dimitrios K PapadopoulosWalter J Gehring
Aug 24, 2010·Developmental Biology·Dianne DuncanIan Duncan
Jun 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Dimitrios K PapadopoulosWalter J Gehring
Dec 14, 2011·Cell·Matthew SlatteryRichard S Mann
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Katherine M LelliRichard S Mann
Citations
Jan 22, 2013·Biology Open·Wei WangJohn H Yoder
Jun 2, 2015·Oncogene·C T Collins, J L Hess
Aug 5, 2015·Mechanisms of Development·Mehdi SaadaouiCorinne Maurel-Zaffran
Oct 3, 2015·Mechanisms of Development·Dimitrios K PapadopoulosVladana Vukojević
May 13, 2014·The FEBS Journal·Han-Ni ChuangHuichin Pan
Oct 16, 2015·Chromosoma·Thomas R Bürglin, Markus Affolter
Feb 12, 2014·Mechanisms of Development·Robert A DrewellJacqueline M Dresch
Jun 3, 2014·Developmental Biology·Peter Nagui RefkiAbderrahman Khila
Oct 22, 2016·Current Opinion in Genetics & Development·Nicoletta Bobola, Samir Merabet
Oct 16, 2018·PloS One·Claudia Altamirano-TorresDiana Reséndez-Pérez
Mar 14, 2014·G3 : Genes - Genomes - Genetics·Lovesha Sivanantharajah, Anthony Percival-Smith
Aug 9, 2015·The Journal of Biological Chemistry·Meng MengQing-You Xia
Mar 25, 2017·Journal of Experimental Botany·Yonghua JiangXiaolin Ren
Nov 25, 2018·International Journal of Molecular Sciences·Zhaojiang GuoYoujun Zhang
Jul 27, 2014·Development·Huai Deng, Tom K Kerppola