Dimeric chlorite dismutase from the nitrogen-fixing cyanobacterium Cyanothece sp. PCC7425

Molecular Microbiology
Irene SchaffnerChristian Obinger

Abstract

It is demonstrated that cyanobacteria (both azotrophic and non-azotrophic) contain heme b oxidoreductases that can convert chlorite to chloride and molecular oxygen (incorrectly denominated chlorite 'dismutase', Cld). Beside the water-splitting manganese complex of photosystem II, this metalloenzyme is the second known enzyme that catalyses the formation of a covalent oxygen-oxygen bond. All cyanobacterial Clds have a truncated N-terminus and are dimeric (i.e. clade 2) proteins. As model protein, Cld from Cyanothece sp. PCC7425 (CCld) was recombinantly produced in Escherichia coli and shown to efficiently degrade chlorite with an activity optimum at pH 5.0 [kcat 1144 ± 23.8 s(-1), KM 162 ± 10.0 μM, catalytic efficiency (7.1 ± 0.6) × 10(6) M(-1) s(-1)]. The resting ferric high-spin axially symmetric heme enzyme has a standard reduction potential of the Fe(III)/Fe(II) couple of -126 ± 1.9 mV at pH 7.0. Cyanide mediates the formation of a low-spin complex with k(on)  = (1.6 ± 0.1) × 10(5) M(-1) s(-1) and k(off) = 1.4 ± 2.9 s(-1) (KD ∼ 8.6 μM). Both, thermal and chemical unfolding follows a non-two-state unfolding pathway with the first transition being related to the release of the prosthetic group. The obtained data are discussed...Continue Reading

References

Nov 1, 1996·Archives of Microbiology·C G van GinkelS W Kengen
Jul 15, 1997·Analytical Biochemistry·D R DoergeM I Churchwell
Nov 11, 1999·Archives of Environmental Contamination and Toxicology·H UenoK Nakamuro
Jul 27, 2001·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·K StenkloT Nilsson
Jan 5, 2002·Journal of the American Chemical Society·Gianantonio BattistuzziMarco Sola
Mar 6, 2004·Archives of Biochemistry and Biophysics·Gianantonio BattistuzziMarco Sola
Mar 23, 2004·Physiologia Plantarum·G. A. PeschekM. Paumann
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jun 16, 2004·Nature Reviews. Microbiology·John D Coates, Laurie A Achenbach
May 3, 2005·Biochimica Et Biophysica Acta·Martina PaumannGünter A Peschek
Jul 5, 2005·BMC Bioinformatics·Jannick Dyrløv BendtsenSøren Brunak
Sep 29, 2005·Journal of Magnetic Resonance·Stefan Stoll, Arthur Schweiger
Oct 11, 2005·BMC Microbiology·Jannick D BendtsenSøren Brunak
May 13, 2006·FEMS Microbiology Ecology·Milagros ZaballosFrancisco Rodríguez-Valera
Jan 9, 2009·Journal of Experimental Botany·Margit BernroitnerChristian Obinger
Feb 21, 2009·FEMS Microbiology Letters·Farrakh MehboobServé W M Kengen
Apr 14, 2009·Journal of Molecular Biology·Daniël C de GeusJan Pieter Abrahams
Dec 1, 2009·Archives of Biochemistry and Biophysics·Gianantonio BattistuzziChristian Obinger
Apr 2, 2010·Journal of the American Chemical Society·Bennett R StreitJennifer L DuBois
Jun 15, 2010·The Journal of Biological Chemistry·Tamara A DaileyHarry A Dailey
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Jun 12, 2012·Biochimica Et Biophysica Acta·Stefan HofbauerChristian Obinger
Aug 7, 2013·The Journal of Biological Chemistry·Markus AuerChristian Obinger
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Feb 13, 2014·Biotechnology Journal·Stefan HofbauerChristian Obinger
May 23, 2014·Journal of Proteome Research·Uma K AryalLouis A Sherman
Dec 17, 2014·Biochimica Et Biophysica Acta·Rosa María Martínez-EspinosaMaría José Bonete
Jan 21, 2015·Archives of Biochemistry and Biophysics·Stefan HofbauerPaul G Furtmüller

❮ Previous
Next ❯

Citations

Aug 20, 2015·The Journal of Physical Chemistry. B·Amy De SchutterSabine Van Doorslaer
Jan 13, 2015·Archives of Biochemistry and Biophysics·Marcel ZámockýChristian Obinger
Jan 21, 2015·Archives of Biochemistry and Biophysics·Stefan HofbauerPaul G Furtmüller
Mar 18, 2015·Archives of Biochemistry and Biophysics·Arianna I Celis, Jennifer L DuBois
Aug 4, 2018·The Journal of Biological Chemistry·Vera PfanzaglChristian Obinger
Jan 9, 2019·Frontiers in Microbiology·Siavash AtashgahiDetmer Sipkema
Feb 10, 2016·Bioscience Reports·Stefan HofbauerChristian Obinger
Oct 29, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Durga MahorPeter-Leon Hagedoorn
Sep 7, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Stefan HofbauerPaul G Furtmüller
Feb 7, 2018·Biochemistry·Zachary GeeraertsGudrun S Lukat-Rodgers

❮ Previous
Next ❯

Methods Mentioned

BETA
circular
electron
electrophoresis
circular dichroism
light scattering
gel filtration
differential scanning calorimetry
proteomic profiling
Fluorescence

Software Mentioned

EasySpin
Pro Data Viewer
MUSCLE
Microcal Origin
MEGA
TatP
Applied Photophysics
VP
MEGA6
viewer

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.