Dimerization and DNA-dependent aggregation of the Escherichia coli nucleoid protein and chaperone CbpA.

Molecular Microbiology
Sarah CosgriffDavid C Grainger

Abstract

The Escherichia coli curved DNA-binding protein A (CbpA) is a nucleoid-associated DNA-binding factor and chaperone that is expressed at high levels as cells enter stationary phase. Using a combination of genetics, biochemistry, structural modelling and single-molecule atomic force microscopy we have examined dimerization of, and DNA binding by, CbpA. Our data show that CbpA dimerization is driven by a hydrophobic surface comprising amino acid side chains W287 and L290 located on the same side of an α helix close to the C-terminus of CbpA. Derivatives of CbpA that are unable to dimerize are also unable to bind DNA. Free in solution, CbpA can exist as either a monomer or dimer. However, when bound to DNA, CbpA forms large aggregates that can protect DNA from degradation by nucleases. These CbpA-DNA aggregates are similar in morphology to protein-DNA complexes formed by the DNA-binding protein from starved cells (Dps), the only other stationary phase-specific nucleoid protein. Conversely, protein-DNA complexes formed by Fis, the major growth phase nucleoid protein, have a markedly different appearance.

References

Sep 1, 1990·Journal of Biochemistry·H YamadaT Mizuno
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·C UeguchiT Mizuno
Apr 18, 1998·Nature Structural Biology·R A GrantJ M Hogle
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·G KarimovaD Ladant
Jul 14, 1999·Nature·S G WolfA Minsky
Aug 18, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T A AzamA Ishihama
Jan 29, 2002·Nucleic Acids Research·R SchneiderG Muskhelishvili
Jun 9, 2004·The Journal of Biological Chemistry·Chi ChaeSue Wickner
May 16, 2006·Journal of Structural Biology·Steven B Zimmerman
Sep 12, 2006·Nucleic Acids Research·David C GraingerStephen J W Busby
Sep 16, 2006·The Journal of Biological Chemistry·Jeremy G BirdSue Wickner
Nov 10, 2006·The EMBO Journal·Ryosuke L OhniwaKunio Takeyasu
Mar 6, 2007·Journal of Bacteriology·Matthew R ChenowethSue Wickner
May 3, 2008·Molecular Microbiology·David C GraingerStephen J W Busby
Feb 28, 2009·Advances in Applied Microbiology·Charles J Dorman
Feb 9, 2010·Nature Reviews. Microbiology·Shane C Dillon, Charles J Dorman

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Citations

Jan 24, 2013·PLoS Genetics·Kiran ChintakayalaDavid C Grainger
Sep 14, 2014·Nucleic Acids Research·Jarmila HnilicováLibor Krásný
Jun 12, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Dominique DurandSophie Quevillon-Cheruel
Jun 21, 2011·Journal of Molecular Biology·Kiran Chintakayala, David C Grainger
Feb 4, 2011·Current Opinion in Microbiology·Sylvie Rimsky, Andrew Travers
Jan 30, 2013·Biochemical Society Transactions·Rosalie P C Driessen, Remus Th Dame
Feb 12, 2015·Nucleic Acids Research·Kiran ChintakayalaDavid C Grainger
Sep 14, 2019·Molecular Microbiology·Rachel A KettlesDavid C Grainger
Feb 20, 2020·Microorganisms·Simona PepeDavide Roncarati
Nov 27, 2019·Nature Reviews. Genetics·Remus T DameDavid C Grainger
Jul 14, 2012·Reports on Progress in Physics·Vincenzo G BenzaMarco Cosentino Lagomarsino
Mar 10, 2019·Biochemical Society Transactions·Anna LankesterDavid C Grainger

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Methods Mentioned

BETA
electron microscopy
atomic force microscopy
AFM
two-hybrid
Bacterial
electrophoretic mobility shift
PCR
electrophoresis

Software Mentioned

NanoScope
WSxM

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