Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity.

Proceedings of the National Academy of Sciences of the United States of America
Athanasios PaschosChristian Baron

Abstract

VirB8-like proteins are essential components of type IV secretion systems, bacterial virulence factors that mediate the translocation of effector molecules from many bacterial pathogens into eukaryotic cells. Based on cell biological, genetic, and x-ray crystallographic data, VirB8 was proposed to undergo multiple protein-protein interactions to mediate assembly of the translocation machinery. Here we report the results of a structure-function analysis of the periplasmic domain of VirB8 from the mammalian pathogen Brucella suis, which identifies amino acid residues required for three protein-protein interactions. VirB8 variants changed at residues proposed to be involved in dimerization, and protein-protein interactions were purified and characterized in vitro and in vivo. Changes at M102, Y105, and E214 affected the self-association as measured by analytical ultracentrifugation and gel filtration. The interaction with B. suis VirB10 was reduced by changes at T201, and change at R230 inhibited the interaction with VirB4 in vitro. The in vivo functionality of VirB8 variants was determined by complementation of growth in macrophages by a B. suis virB8 mutant and by using a heterologous assay of type IV secretion system assembly i...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·R Valdes, G K Ackers
Mar 1, 1994·Journal of Bacteriology·Y R Thorstenson, P C Zambryski
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BohneA N Binns
Jul 31, 1999·The Journal of Biological Chemistry·R EisenbrandtE Lanka
May 24, 2001·Journal of Bacteriology·V SagulenkoP J Christie
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Maria Laura BoschiroliDavid O'Callaghan
Feb 16, 2002·Journal of Molecular Biology·C Chaillan-HuntingtonM Stewart
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Doyle V WardPatricia C Zambryski
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lilian KrallChristian Baron
Feb 20, 2004·Journal of Bacteriology·Michael E Hudson, Justin R Nodwell
May 25, 2004·Science·Eric Cascales, Peter J Christie
Jul 3, 2004·Journal of Bacteriology·Alireza Shamaei-TousiGad Frankel
Jul 23, 2004·Current Molecular Medicine·Felix Hoppe-SeylerKarin Butz
Nov 18, 2004·Biochimica Et Biophysica Acta·Peter J Christie
Dec 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric Cascales, Peter J Christie
Mar 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Laurent TerradotGabriel Waksman
May 20, 2005·The Journal of Biological Chemistry·Qing YuanChristian Baron
May 25, 2005·Annual Review of Microbiology·John A Fuerst
Aug 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Paul K JuddAnath Das
Sep 13, 2005·Annual Review of Microbiology·Georges N Cohen
Sep 13, 2005·Annual Review of Microbiology·Peter J ChristieEric Cascales
Nov 29, 2005·Journal of Psychosomatic Research·Caroline E WrightJohn Weinman
Feb 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Susan BaileyPatricia C Zambryski

❮ Previous
Next ❯

Citations

Apr 2, 2013·Research in Microbiology·Minny BhattyPeter J Christie
Apr 30, 2010·The Journal of Biological Chemistry·Yun-Long TsaiErh-Min Lai
Jan 12, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Christian Baron
Aug 27, 2014·Future Microbiology·David SteadmanHan Remaut
Jan 19, 2010·Current Opinion in Microbiology·Christian Baron
Jan 30, 2013·Biochemical Society Transactions·Vidya Chandran
Jan 7, 2014·Biochimica Et Biophysica Acta·Peter J ChristieChristian González-Rivera
Jun 4, 2015·Annual Review of Biochemistry·Vidya Chandran Darbari, Gabriel Waksman
Apr 22, 2015·The American Journal of Pathology·Paul de FigueiredoL Garry Adams
Apr 23, 2016·Scientific Reports·Christian FercherWalter Keller
Aug 28, 2012·Chemistry & Biology·Mark A SmithChristian Baron
Jun 17, 2016·Pathogens and Disease·Joseph J GillespieAbdu F Azad
Oct 14, 2016·Frontiers in Cellular and Infection Microbiology·Pedro F Soler-LlorénsAnne Keriel
Aug 3, 2017·FEBS Letters·Mahzad SharifahmadianChristian Baron
Sep 17, 2016·The Journal of Biological Chemistry·Bastien CasuChristian Baron
Jun 6, 2019·Frontiers in Microbiology·Germán G SgroChuck S Farah
May 23, 2018·Proceedings of the National Academy of Sciences of the United States of America·Bastien CasuChristian Baron
Feb 12, 2021·Microbiology and Molecular Biology Reviews : MMBR·R Martin RoopDaniel W Martin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.