Dimerization of matrix metalloproteinase-2 (MMP-2): functional implication in MMP-2 activation.

The Journal of Biological Chemistry
Bon-Hun KooDoo-Sik Kim

Abstract

Matrix metalloproteinase-2 (MMP-2) functions in diverse biological processes through the degradation of extracellular and non-extracellular matrix molecules. Because of its potential for tissue damage, there are several ways to regulate MMP-2 activity, including gene expression, compartmentalization, zymogen activation, and enzyme inactivation by extracellular inhibitors. Enzyme regulation through zymogen activation is important for the regulation of MMP-2 activity. In our previous studies, we showed that thrombin directly cleaved the propeptide of MMP-2 at specific sites for enzyme activation. We also demonstrated that heparan sulfate was required for thrombin-mediated activation of pro-MMP-2 by binding to thrombin, presumably through conformational changes at the active site of the enzyme. This suggests a regulatory mechanism for thrombin-mediated activation of pro-MMP-2. In this study, we found that MMP-2 formed a reduction-sensitive homodimer in a controlled manner and that Ca(2+) ion was essential for homodimerization of MMP-2. Homodimerization was not associated with protein kinase C-mediated phosphorylation of MMP-2. MMP-2 formed a homodimer through an intermolecular disulfide bond between Cys(102) and the neighboring Cy...Continue Reading

References

Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Mar 15, 1989·Biochemical and Biophysical Research Communications·E KobayashiT Tamaoki
Sep 29, 1989·Biochemical and Biophysical Research Communications·K R Rao, K Brew
Sep 6, 1994·Annals of the New York Academy of Sciences·J F Woessner
Jun 1, 1994·Journal of Cellular Biochemistry·K Oishi, M Yamaguchi
Mar 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·Z S GalisP Libby
Sep 22, 1998·The Biochemical Journal·J M ChenA J Barrett
Sep 28, 1998·Biochimica Et Biophysica Acta·A C Newton, J E Johnson
Sep 24, 1999·Biochemical and Biophysical Research Communications·A BescondP Charpiot
Nov 1, 2000·Cell·J Schlessinger
Oct 5, 2001·The Journal of Biological Chemistry·C J MorrisonC M Overall
Jul 20, 2002·Journal of Molecular Biology·Hyunju ChaHans Brandstetter
Dec 14, 2002·Biochemical and Biophysical Research Communications·Seiji FujisakiMasamoto Kanno
Jun 13, 2003·Genome Biology·Robert P T SomervilleSuneel S Apte
Aug 2, 2003·Archives of Biochemistry and Biophysics·M R CominettiH S Selistre-de-Araujo
Aug 18, 2004·Biotechnology and Applied Biochemistry·Razieh Yazdanparast, Reza Khodarahmi
Jan 13, 2006·Cardiovascular Research·Hideaki NagaseGillian Murphy
Dec 26, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Neil HendersonSimon Richard Johnson
Mar 14, 2007·Stem Cells and Development·Yoshie OzakiYukio Kato
Apr 17, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Meltem SariahmetogluRichard Schulz
Aug 3, 2007·Matrix Biology : Journal of the International Society for Matrix Biology·Hyun-Jeong Ra, William C Parks
Feb 28, 2009·Arthritis and Rheumatism·Miriam T JacksonChristopher B Little
Jul 8, 2009·The Journal of Biological Chemistry·Bon-Hun KooDoo-Sik Kim
Nov 3, 2010·The Journal of Biological Chemistry·Bon-Hun KooDoo-Sik Kim

❮ Previous
Next ❯

Citations

Jan 31, 2013·The Journal of Biological Chemistry·Jennifer L MeitzlerPaul R Ortiz de Montellano
Feb 15, 2015·Basic Research in Cardiology·Anton BaysaGuro Valen
May 7, 2016·Expert Reviews in Molecular Medicine·Laura BianchiPaola Piomboni
Mar 14, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ann Iren SolliElin Hadler-Olsen
Jul 22, 2014·Chembiochem : a European Journal of Chemical Biology·Kamila FilipiakBeatriz de Pascual-Teresa
Jun 18, 2017·Journal of the American Heart Association·Antoine H ChaanineMargaret M Redfield
Dec 17, 2015·The Journal of Biological Chemistry·Hansen J KosasihAmanda J Fosang
Jan 1, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Bryan G HughesRichard Schulz
Aug 5, 2015·Journal of Forensic Sciences·Yenan WangFukumi Furukawa
May 1, 2021·International Journal of Molecular Sciences·Matthew Thomas FerreiraAlison Colquhoun

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.