Dimerization of MoMuLV genomic RNA: redefinition of the role of the palindromic stem-loop H1 (278-303) and new roles for stem-loops H2 (310-352) and H3 (355-374)

Biochemistry
M De TapiaC Ehresmann

Abstract

Genomic RNAs from retroviruses are packaged as dimers of two identical RNA molecules. In Moloney murine leukemia virus, a stem-loop structure (H1) located in the encapsidation domain Psi (nucleotides 215-564) was postulated to trigger RNA dimerization through base pairing between auto complementary sequences. The Psi domain also contains two other stem-loop structures (H2 and H3) that are essential for RNA packaging. Since it was suspected than H1 is not the only element involved in RNA dimerization, we systematically investigated the dimerization capacity of several subdomains of the first 725 nucleotides of genomic RNA. The efficiency of dimerization of the various RNAs was estimated by measuring their apparent dissociation constants, and the specificity was tested by competition experiments. Our results indicate that the specificity of dimerization of RNA nucleotides 1-725 is driven by motifs H1-H3 in domain Psi. To define the relative contributions of these elements, RNA deletion mutants containing different combinations of H1-H3 were constructed and further analyzed in competition and kinetic experiments. Our results confirm the importance of H1 in triggering dimerization and shed new light on the mechanism of dimerization...Continue Reading

References

Oct 1, 1996·Journal of Virology·B Berkhout, J L van Wamel
Nov 15, 1996·The Journal of Biological Chemistry·E SkripkinC Ehresmann

❮ Previous
Next ❯

Citations

Mar 9, 2004·Journal of Molecular Biology·Victoria D'SouzaMichael F Summers
Jun 11, 2002·Journal of Molecular Biology·Jean-Marc Lanchy, J Stephen Lodmell
Nov 30, 2002·Biochimie·Christine BrunelChantal Ehresmann
Aug 3, 2005·Nature Reviews. Microbiology·Victoria D'Souza, Michael F Summers
May 21, 2004·Nature Reviews. Microbiology·Jean-Christophe PaillartJohnson Mak
Jan 13, 2004·Nucleic Acids Research·Jacob Giehm MikkelsenFinn Skou Pedersen
Mar 9, 2002·Journal of Virology·Hinh Ly, Tristram G Parslow
Apr 5, 2002·Journal of Virology·Elena Izmailova, Anna Aldovini
Sep 29, 2004·Journal of Virology·Catherine S HibbertAlan Rein
Oct 28, 2004·Journal of Virology·Jessica A FlynnAlice Telesnitsky
Sep 4, 2004·Retrovirology·Rodney S RussellMark A Wainberg
Aug 7, 2010·Retrovirology·Stéphan Maurel, Marylène Mougel
Jan 16, 2010·RNA·Sergei Kharytonchyk, Finn Skou Pedersen
Oct 25, 2000·Photochemistry and Photobiology·Y HuiR Carpentier
Aug 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·C H Kim, I Tinoco
Oct 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Pan T X LiIgnacio Tinoco
Jan 13, 2006·Nature Chemical Biology·Christopher S Badorrek, Kevin M Weeks
Oct 7, 2010·International Reviews of Immunology·Alexander P GultyaevRené C L Olsthoorn
Oct 12, 2010·Journal of Molecular Biology·Yasuyuki MiyazakiMichael F Summers
Jul 15, 2015·RNA Biology·Lucie Pessel-VivaresMarylène Mougel
Nov 18, 2005·Journal of Molecular Biology·Fatima SmagulovaLaurent Houzet
Oct 29, 2005·Journal of Molecular Biology·Eugenia BasyukSøren Vestergaard Rasmussen
Nov 26, 2009·Journal of Molecular Biology·Yasuyuki MiyazakiMichael F Summers
Jun 22, 2011·RNA Biology·Nolwenn JouvenetMarylène Mougel
Aug 17, 1999·Journal of Molecular Biology·E M OroudjevL A Kohlstaedt
Sep 21, 2006·The Journal of Biological Chemistry·Cristina Gherghe, Kevin M Weeks
Nov 28, 2000·The Journal of Biological Chemistry·F JossinetR Marquet
Apr 7, 2018·Frontiers in Microbiology·Noé DuboisSerena Bernacchi
Jul 19, 2006·The Journal of General Virology·Søren Vestergaard Rasmussen, Finn Skou Pedersen
Oct 4, 2020·Viruses·Patricia S BoydSaif Yasin
Jan 9, 2017·Biochemical and Biophysical Research Communications·Wally ChuChul-Hyun Kim
Jun 23, 2000·Journal of Molecular Biology·E PolgeP Fossé

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.