Dimerization through the RING-Finger Domain Attenuates Excision Activity of the piggyBac Transposase

Biochemistry
Rahul SharmaDeepak T Nair

Abstract

The movement of the piggyBac transposon is mediated through its cognate transposase. The piggyBac transposase binds to the terminal repeats present at the ends of the transposon. This is followed by excision of the transposon and release of the nucleoprotein complex. The complex translocates, followed by integration of the transposon at the target site. Here, we show that the RING-finger domain (RFD) present toward the C-terminus of the transposase is vital for dimerization of this enzyme. The deletion of the RFD or the last seven residues of the RFD results in a monomeric protein that binds the terminal end of the transposon with nearly the same affinity as wild type piggyBac transposase. Surprisingly, the monomeric constructs exhibit >2-fold enhancement in the excision activity of the enzyme. Overall, our studies suggest that dimerization attenuates the excision activity of the piggyBac transposase. This attribute of the piggyBac transposase may serve to prevent excessive transposition of the piggyBac transposon that might be catastrophic for the host cell.

References

Jan 1, 1984·Methods in Enzymology·C R MerrilM L Van Keuren
Feb 9, 1984·Nature·W F DoolittleM A Dempster
Jun 11, 1993·Annals of the New York Academy of Sciences·P S Freemont
Oct 1, 1996·Nucleic Acids Research·D York, W S Reznikoff
May 29, 2000·The Journal of Biological Chemistry·M Steiniger-White, W S Reznikoff
Sep 28, 2000·Cell·C A Joazeiro, A M Weissman
Feb 13, 2003·Journal of the American Chemical Society·Cyril DominguezAlexandre M J J Bonvin
Apr 1, 2003·IUBMB Life·Jacqueline M Matthews, Margaret Sunde
Apr 16, 2003·Cell Cycle·L AravindE V Koonin
Jul 5, 2005·Journal of Molecular Biology·Corinne Augé-GouillouYves Bigot
Jul 26, 2005·Nature Structural & Molecular Biology·Alison B HickmanFred Dyda
Jan 11, 2007·Trends in Biochemical Sciences·Roland GamsjaegerJoel P Mackay
Mar 18, 2008·Insect Biochemistry and Molecular Biology·Jianjun WangYoonseong Park
Mar 21, 2008·The EMBO Journal·Rupak MitraNancy L Craig
Apr 19, 2008·Nature Reviews. Genetics·Vladimir V Kapitonov, Jerzy Jurka
May 26, 2009·Nature Structural & Molecular Biology·Qian YinHao Wu
May 1, 2010·Nature Protocols·Sjoerd J de VriesAlexandre M J J Bonvin
Aug 21, 2010·Genome Génome / Conseil National De Recherches Canada·Takaaki DaimonToru Shimada
Mar 29, 2011·Current Opinion in Structural Biology·Sherwin P Montaño, Phoebe A Rice
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Nov 9, 2012·Nature·Sherwin P MontañoPhoebe A Rice
Jun 26, 2013·ELife·Corentin Claeys BouuaertRonald Chalmers
Feb 28, 2014·Methods in Molecular Biology·Gydo C P van Zundert, Alexandre M J J Bonvin
Mar 27, 2015·Current Cancer Drug Targets·Benoit Chenais
Jul 28, 2015·Trends in Biotechnology·Lauren E Woodard, Matthew H Wilson
Sep 28, 2015·Journal of Molecular Biology·G C P van ZundertA M J J Bonvin
Dec 3, 2016·Nucleic Acids Research·Lauren E WoodardMatthew H Wilson
Jan 14, 2017·Genome Biology and Evolution·Maryem BouallèguePierre Capy

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Citations

Jan 16, 2021·Journal of Molecular Biology·Laura HelouYves Bigot

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