PMID: 25730801Mar 3, 2015Paper

Dimethyl 3, 3', 4, 4'-tetrahydroxy-δ-truxinate isolated from the leaves of Andrographis lineata.Wall. ex. Nees suppress adipogenesis in 3T3-L1 preadipocytes for type 2 diabetes

Pakistan Journal of Pharmaceutical Sciences
Vijayakumar Sudarshana DeepaPeriyasamy Sureshkumar

Abstract

The present investigation elucidates the isolation and characterization of bioactive compound from the ethanolic leaf extract of Andrographis lineata (EtALL) which suppress the differentiation of 3T3-L1 adipocytes. The ethanolic leaf extract was subjected to bioassay guided fractionation in 3T3-L1 cell lines. Five fractions were isolated from the EtALL extract by column chromatography. All the Fractions (I-V) along with EtALL were screened for adipogenesis activity (Oil-Red-O staining).The fraction which showed maximum adipogenesis activity was purified by thin layer chromatography. The bioactive Fraction IV was found to have maximum adipogenic (96.83%) activity and the activity was comparable to Rosiglitazone. The spectroscopic data analysis reveals that, the isolated bioactive compound was Dimethyl 3, 3', 4, 4'-tetrahydroxy-δ-truxinate (DTδT), a combination of truxillic and truxinic acid derivative. DTδT showed insulin mimicking (131.2%), sensitizing (810.02%) and adipogenic activity (80.23%). Hence our present study concluded that, Dimethyl 3, 3', 4, 4'-tetrahydroxy-δ-truxinate isolated from the ethanolic leaf extract of Andrographis lineata stimulates glucose uptake, potentiates insulin-stimulated glucose in 3T3-L1 adipocyt...Continue Reading

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