Diminished adherence of snail hemocytes to schistosome sporocysts of Schistosoma mansoni following programmed knockout of the allograft inflammatory factor of Biomphalaria glabrata

BioRxiv : the Preprint Server for Biology
F. S. CoelhoW. I. Tanno

Abstract

Schistosomiasis mansoni is a debilitating neglected tropical disease caused by infection with the blood fluke, Schistosoma mansoni. Development of the larval stage of the parasite in an intermediate host snail of the genus Biomphalaria is an obligatory component of the life cycle. Enhanced understanding of the mechanism(s) of host defense in the intermediate host snail may hasten the development of new tools that block transmission of schistosomiasis. The embryonic cell line from B. glabrata, termed the Bge line, is a versatile resource in the investigation of snail-schistosome interactions. A key attribute of the Bge cell is its hemocyte-like phenotype, given the central role of the snail hemocyte in innate and cellular immunity. The allograft inflammatory factor 1 (AIF, aif) is a conserved antigen typically expressed in phagocytic and granular leukocytes and is a marker of macrophage activation. AIF is highly expressed in isolates of B. glabrata that are resistant to infection with S. mansoni. We targeted the aif gene of B. glabrata (BgAIF) for programmed gene knockout using the CRISPR/Cas9 approach, to investigate the activity of CRISPR/Cas9 gene editing in this gastropod species and, in turn, to investigate the loss of AIF ...Continue Reading

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