Dinuclear ruthenium complexes display loop isomer selectivity to c-MYC DNA G-quadriplex and exhibit anti-tumour activity

Journal of Inorganic Biochemistry
Chuping ZhengJie Liu

Abstract

G-quadruplex DNA, especially the cellular-myelocytomatosis viral oncogene (c-MYC) is closely associated with cell-cycle regulation, proliferation of tumour cells. In this work, the interaction between the c-MYC and two dinuclear Ru(II) complexes [(bpy)2Ru(bpibp)Ru(bpy)2](ClO4)4 (compound 1) and [(phen)2Ru(bpibp)Ru(phen)2](ClO4)4 (compound 2) have been studied. The data from UV-Visible, PCR-stop and Fluorescence resonance energy transfer (FRET) showed that two complexes can stabilize the structure of G-quadruplex in the c-MYC promoter and targeting the G-quadruplex loop isomers. Interestingly, the complex 2 has a greater effect on the 1:2:1 and 2:1:1 loop isomers while the 1 prefers to the 1:2:1 isomers. The mechanism studies revealed that complexes can induce apoptosis in HepG2 cells by generating ROS metabolites, triggering mitochondrial membrane potential loss and down-regulation of P-Akt (Akt also known as protein kinase B), P-p44/42 MAP kinase protein (P-p44/42), and c-MYC. Taken together, these results suggested that the two dinuclear complexes may both be candidates as anti-tumour agents as they may reduce the c-MYC gene expression. {bpibp: 4, 4'-bis (1, 10-phenanthroline-[5, 6-d] imidazole-2-yl)-biphenyl, bpy: 2,2-bipyri...Continue Reading

References

Apr 29, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·F Labat-MoleurA Negoescu
Apr 19, 2002·Biochimica Et Biophysica Acta·Werner LutzMartin Eilers
Jun 7, 2002·Nature·Gary N ParkinsonStephen Neidle
Oct 17, 2008·Chembiochem : a European Journal of Chemical Biology·Anne De CianPatrizia Alberti
Nov 30, 2010·Genes & Cancer·Tracy A Brooks, Laurence H Hurley
Jan 4, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Chuanqi ZhaoXiaogang Qu

❮ Previous
Next ❯

Citations

Jun 1, 2018·International Journal of Molecular Sciences·Maiara de Souza OliveiraDaniel Pereira Bezerra

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Dalton Transactions : an International Journal of Inorganic Chemistry
Xiang ChenLiang-Nian Ji
Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Chuan WangJie Liu
Archiv der Pharmazie
Rajendran Senthil KumarMohammad Abdulkadher Akbarsha
© 2022 Meta ULC. All rights reserved