PMID: 9166752Jun 1, 1997Paper

Dinucleotide receptor modulation by protein kinases (protein kinases A and C) and protein phosphatases in rat brain synaptic terminals

Journal of Neurochemistry
J PintorM T Miras-Portugal

Abstract

The diadenosine polyphosphates, diadenosine tetraphosphate and diadenosine pentaphosphate (Ap5A), can activate an ionotropic dinucleotide receptor that induces Ca2+ transients into synaptosomes prepared from rat brain. This receptor, also termed the P4 purinoceptor, is sensitive only to adenine dinucleotides and is insensitive to ATP. Studies on the modulatory role of protein kinase A (PKA), protein kinase C (PKC), and protein phosphatases on the response of diadenosine polyphosphate receptors were performed by measuring the changes in the intracellular Ca2+ levels with fura-2. Activation and inhibition of PKA were carried out by means of forskolin and the PKA inhibitory peptide (PKA-IP), respectively. The Ap5A response was inhibited by forksolin to 35% of control values, but PKA-IP induced an increase of 37%. The effect of PKC activation was similar to that observed for PKA. PKC stimulation with phorbol 12,13-dibutyrate produced an inhibition of 67%, whereas the PKC inhibitors staurosporine and PKC inhibitory peptide enhanced the responses elicited by Ap5A to 40% in both cases. Protein phosphatase inhibitors diminished the responses elicited by Ap5A to 17% in the case of okadaic acid, to 50% for microcystin, and to 45% in the ...Continue Reading

Citations

May 12, 2000·British Journal of Pharmacology·M Díaz-HernándezM T Miras-Portugal
Jun 3, 2000·Journal of Cardiovascular Pharmacology·N ZimmermannE Gams
Sep 13, 2003·Journal of Neurochemistry·Javier GualixM Teresa Miras-Portugal
May 12, 2006·Pflügers Archiv : European journal of physiology·Esmerilda G DelicadoJavier Gualix
Apr 29, 2004·Nucleosides, Nucleotides & Nucleic Acids·Luminita H PojogaRichard H Hilderman
Jul 25, 1998·FEBS Letters·M T Miras-PortugalJ Pintor
Apr 19, 2002·The Journal of Pharmacology and Experimental Therapeutics·Miguel Díaz-HernándezMaría Teresa Miras-Portugal
Jul 1, 2009·British Journal of Pharmacology·Vera JankowskiJoachim Jankowski
Mar 19, 1999·European Journal of Pharmacology·J PintorM T Miras-Portugal
Sep 29, 2000·Pharmacology & Therapeutics·J PintorM T Miras-Portugal
Jun 28, 2000·Journal of the Autonomic Nervous System·M T Miras-PortugalJ Pintor
Feb 27, 2003·Neuropharmacology·R Gómez-VillafuertesM T Miras-Portugal

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