Diol appended quenchers for fluorescein boronic acid

Chemistry, an Asian Journal
Souad A ElfekyJohn S Fossey

Abstract

Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Förster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.

References

Dec 4, 2003·Bioorganic & Medicinal Chemistry Letters·David EgronChristophe Len
Dec 16, 2003·Angewandte Chemie·Jeff T SuriBakthan Singaram
Feb 16, 2008·Nature Protocols·Yolanda Pérez-FuertesTony D James
Feb 16, 2008·Nature Protocols·Andrew M KellyTony D James
Mar 14, 2008·The Journal of Organic Chemistry·David K ScraftonTony D James
Jun 21, 2008·Chemistry, an Asian Journal·Norifumi FujitaTony D James
Oct 18, 2008·Electrophoresis·Thomas R JacksonJean M H van den Elsen
Mar 14, 2009·Chemical Communications : Chem Comm·Winson M J MaJohn S Fossey
Mar 19, 2009·Chemical Communications : Chem Comm·Kenichi KataokaYuji Kubo
Nov 10, 2009·Proteomics·Marta P Pereira MoraisJean M H van den Elsen
Mar 20, 2010·Chemical Reviews·Jacques LebretonChristophe Len

❮ Previous
Next ❯

Citations

May 15, 2015·Chemical Reviews·Xiaolong Sun, Tony D James
Mar 28, 2013·Scientific Reports·Marta P Pereira MoraisJean M H van den Elsen
Jul 14, 2012·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·John S FosseyTony D James
Nov 15, 2012·Accounts of Chemical Research·Steven D BullTony D James
Jun 9, 2017·Chemistry, an Asian Journal·Arnaud ChevalierAnthony Romieu
Dec 1, 2010·Chemical Communications : Chem Comm·Ryuhei NishiyabuJohn S Fossey
Nov 30, 2010·Chemical Communications : Chem Comm·Ryuhei NishiyabuJohn S Fossey
Jan 7, 2019·Journal of Fluorescence·Diana M A CristaJoaquim C G Esteves da Silva

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.