Dioscin attenuates renal ischemia/reperfusion injury by inhibiting the TLR4/MyD88 signaling pathway via up-regulation of HSP70

Pharmacological Research : the Official Journal of the Italian Pharmacological Society
Meng QiJinyong Peng

Abstract

We previously reported the effect of dioscin against hepatic ischemia/reperfusion injury (IRI) in rats. However, little is known concerning the role of dioscin in renal IRI. In the present study, rats were subjected to IRI and dioscin was intragastrically administered for seven consecutive days before surgery. In vitro models of hypoxia/reoxygenation were developed in NRK-52E and HK-2 cells, which were prophylactically treated with or without dioscin. The results showed that dioscin significantly decreased serum BUN and Cr levels, and markedly attenuated cell injury. Mechanistic studies showed that dioscin significantly increased HSP70 levels, decreased the levels of TLR4, MyD88, TRAF6, COX-2, JNK, ERK and p38 MAPK phosphorylation, suppressed the nuclear translocation of NF-κB and HMGB1, and subsequently decreased the mRNA levels of IL-1β, IL-6, TNF-α, ICAM-1 and IFN-γ. Moreover, HSP70 siRNA or TLR4 DNA reversed the nephroprotective effects of dioscin, while dioscin still significantly down-regulated the TLR4 signaling pathway. Furthermore, by inhibiting MyD88 with ST2825 (a MyD88 inhibitor), renal IRI was significantly attenuated, suggesting that the effect of dioscin against renal IRI depended on MyD88. Our results suggested ...Continue Reading

References

Jun 1, 1992·The Journal of Cell Biology·C HardwickE A Turley
Apr 10, 1999·The Journal of Biological Chemistry·J C ChowF Gusovsky
Jul 16, 2004·Kidney International·John J Friedewald, Hamid Rabb
Nov 9, 2004·Trends in Immunology·Alberto MantovaniMassimo Locati
May 25, 2005·Transplantation·Byung Soo KimChul Woo Yang
Sep 15, 2007·The Journal of Clinical Investigation·Huiling WuSteven J Chadban
Feb 5, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Z M El-ZoghbyF G Cosio
May 28, 2009·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·David Sáenz-MoralesMaría Laura García-Bermejo
Feb 24, 2010·Nature Reviews. Nephrology·Anna GlubaJacek Rysz
Jun 25, 2010·Journal of Leukocyte Biology·Subhra K Biswas, Claire E Lewis
Feb 3, 2012·Cold Spring Harbor Perspectives in Biology·Kim Newton, Vishva M Dixit
Feb 16, 2012·Journal of Applied Toxicology : JAT·Mingming HuXianyao Wan
Dec 29, 2012·International Journal of Biological Macromolecules·Guangting CongLirong Hao
Feb 9, 2013·The Journal of Surgical Research·Yi ZhengYunpeng Wang
Feb 12, 2013·Critical Care : the Official Journal of the Critical Care Forum·Norbert LameireUNKNOWN KDIGO AKI Guideline Work Group
Mar 28, 2013·Pharmacology & Therapeutics·Jing LiHang Yin
Aug 16, 2013·American Journal of Physiology. Renal Physiology·Takuya YoshidaNaoki Ikegaya
Oct 18, 2013·American Journal of Physiology. Renal Physiology·Ju-Rong YangYa-Ni He
Dec 10, 2013·Current Opinion in Organ Transplantation·Huiling Wu, Steven J Chadban
Jan 2, 2014·Kidney International·Stephen O'Neill, Jeremy Hughes
Feb 1, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Kristin MorethLiliana Schaefer
Feb 14, 2014·American Journal of Physiology. Renal Physiology·Hailin ZhaoDaqing Ma
Apr 10, 2014·American Journal of Nephrology·Xiaoreng WangXiuhua Liu
Jun 14, 2014·Nephron. Experimental Nephrology·Stephen O'NeillJeremy Hughes
Aug 27, 2014·International Immunopharmacology·Su-Jie JiaMing Zhao

❮ Previous
Next ❯

Citations

Feb 24, 2016·International Immunopharmacology·Luxi Chen, Jianhua Yu
May 27, 2016·Journal of Natural Products·Yun-Lung ChungChieh-Hsi Wu
Jul 20, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Meng QiJinyong Peng
Dec 30, 2017·Molecular Medicine Reports·Nanye WangGuodong Li
Aug 14, 2020·The Journal of International Medical Research·Zhen ZhangXin Chen
Jul 28, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lisha ZhaoJinyong Peng
Sep 13, 2019·BioMed Research International·Longfei YangLufei Wang
Mar 16, 2021·Journal of Ethnopharmacology·Xiaofen LiWenjun Zou
Dec 25, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hua LiangJian Huang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.