Dioscorea japonica extract down-regulates prostaglandin E2 synthetic pathway and induces apoptosis in lung cancer cells

Journal of Clinical Biochemistry and Nutrition
Toshiko Suzuki-YamamotoYoshitaka Takahashi

Abstract

Prostaglandin E2 plays a role in an array of pathophysiological responses, including inflammation, carcinogenesis and so on. Prostaglandin E2 is synthesized from arachidonic acid by the enzymes cyclooxygenase and prostaglandin E synthase. In some pathological conditions, the isozymes cyclooxygenase-2 and microsomal prostaglandin E synthase-1 are transiently induced, leading to prostaglandin E2 overproduction. The present study showed that Dioscorea japonica extract suppresses mRNA expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 in human non-small-cell lung carcinoma A549 cells in a dose-dependent manner. The suppressive effects of Dioscorea japonica extract on the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 were confirmed by Western blotting, cyclooxygenase activity and prostaglandin E2 production. Dioscorea japonica extract induced the translocation of nuclear factor-κB from the nucleus to the cytosol and inhibited the activity of the cyclooxygenase-2 promoter. Furthermore Dioscorea japonica extract suppressed the expression of the anti-apoptotic factor B-cell chronic lymphocytic leukemia/lymphoma 2 and enhanced apoptotic terminal deoxynucleotidyl transferase-mediated dUTP ...Continue Reading

References

Apr 24, 1991·Biochimica Et Biophysica Acta·W L Smith, L J Marnett
Jul 1, 1990·Journal of Neurochemistry·T Shimizu, L S Wolfe
Jan 21, 1993·International Journal of Cancer. Journal International Du Cancer·K Husgafvel-PursiainenH Vainio
Dec 27, 1996·The Journal of Biological Chemistry·W L SmithD L DeWitt
Aug 15, 1998·The Journal of Biological Chemistry·Kotha SubbaramaiahAndrew J Dannenberg
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·P J JakobssonB Samuelsson
Aug 14, 1999·Biochimica Et Biophysica Acta·K WatanabeT Suzuki
Nov 13, 2001·British Journal of Pharmacology·Y S Bakhle
Jan 31, 2002·Annual Review of Medicine·Marco E Turini, Raymond N DuBois
Feb 28, 2002·Biochemical and Biophysical Research Communications·Naomi TanikawaKikuko Watanabe
Jun 28, 2002·Journal of Environmental Pathology, Toxicology and Oncology : Official Organ of the International Society for Environmental Toxicology and Cancer·Takashi TakahashiToyoaki Hida
Oct 16, 2003·Journal of Agricultural and Food Chemistry·Deng-Jye YangLucy Sun Hwang
Jul 30, 2005·International Journal of Cancer. Journal International Du Cancer·Madhu Sudhan ShaikMandip Singh
Jul 7, 2007·Sub-cellular Biochemistry·K D Rainsford
Apr 23, 2009·International Journal of Cancer. Journal International Du Cancer·Sowmyalakshmi SrinivasanChendil Damodaran
May 22, 2009·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Yuki KawakamiYoshitaka Takahashi
Oct 16, 2010·Current Opinion in Gastroenterology·Siew C Ng, Francis K L Chan
Jun 22, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Dae Sung KimYeun Ja Mun

Methods Mentioned

BETA
PCR
protein assay
light microscopy
Assay

Related Concepts

Related Feeds

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.